Required practical 2: culturing microorganisms

Question 1

What is inside of a petri dish?

Answer 1

Agar gel

Question 2

How is bacteria transfered into the petri dish?

Answer 2

A sterilized innoculating loop

Question 3

How do you sterilize an innoculating loop?

Answer 3

Pass it through a flame to kill any unwanted bacteria

Question 4

Why do we incubatebacteria at 25°C instead of 37°C?

Answer 4

To prevent harmful bacteria from growing

Question 5

What is the first step for esting bacterial growth?

Answer 5

Clean work area with disinfectant solution, to prevent contamination. Including sterilizing inncoculating loop by passing it through the bunsen burner.

Question 6

What is the next step after sterilizing the work area?

Answer 6

Open sterile agar gel plate. Ensure it is near a bunsen burner to kill bacteria in the air.

Question 7

Everything is prepared for the practical what is done first?

Answer 7

Now use the innoculating loop to spread the chosen bacteria evenly around the plate.

Question 8

What is done after bacteria is evenly spread?

Answer 8

Place sterile filter paper discs containing chosen antibiotic onto the plate

Question 9

What is the last step of the practical?

Answer 9

Finally incubate the plate at 25°C

Question 10

What is the area where bacteria has not grrown called?

Answer 10

Area of inhibition