Required Practical 1 - Rate of Enzyme-Controlled Reaction (Colorimter Use) Flashcards

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1
Q

Suggest why a colorimeter is used

A
  • provides quantitative result
  • more accurate (not subjective)
  • easier to compare
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2
Q

Suggest why a water bath is used over a Bunsen burner

A
  • stable temperature
  • uniform heating
  • safer since no open flame
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3
Q

Suggest the importance of calibrating a colorimeter using a control

A
  • consistent readings

- container and buffer solution absorb light as well so provides a base line

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4
Q

Suggest why a buffer solution is used in investigations not measuring effect of pH on enzyme activity

A
  • maintains constant pH

- pH is controlled variable

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5
Q

Suggest why it is important never to touch the sides of a cuvette

A

Fingerprints affect the absorbance reading on colorimeter

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6
Q

Suggest why a colourless buffer solution should be used in a colorimeter

A

Coloured buffer solution would affect the absorbance reading on colorimeter

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7
Q

Suggest why solutions are mixed gently before placed in a colorimeter

A

Avoids formation of bubbles or froth which significantly affect absorbance reading on colorimeter

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8
Q

Suggest why test tubes are left in the water bath for a period of time before the reaction

A
  • temperature can equilibrate

- reach temperature for reaction to occur

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9
Q

Suggest why a water bath at room temperature is used

A
  • keeps test tubes at a constant temperature

- room air temperature fluctuates

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10
Q

Describe and explain what you did to make sure the temperatures of the water baths were as reliable as possible

A
  • measure temperature before and after each reaction

- ensure there is little/no change

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