Required Prac 4

Question 1

Student used the same vol of water in each tube explain why that it is important he controlled this

Answer 1

To much water would mean concentration would be lower

So results are comparable

Question 2

How does increased temp cause pigment to be released

Answer 2

Cell membrane damaged
Membrane proteins denatured
Damage to phospholipid bilayer
More pig released

Question 3

A student did not have a colourimeter

Describe a method this student could use to prepare colour standards and use them to give data for the total pigment extracted

Answer 3

1. Use a known conc of extract
2. Prepare a dilution series
3. Compare with colour standards to give a concentration

Question 4

How does ethonol effect pigment released

Answer 4

Damages membrane
Phospholipid bilayer = soluble in ethanol - dissolves
More pigment released