Reproduction Flashcards
How long is the bovine luteal phase?
17 days
Hormonal change at the start of pro-oestrus?
Progesterone is falling
Oestradiol is rising
At what time does peak oestrus occur in cows?
Mid-oestrus during the LH surge (time to AI)
How long after peak oestrus does ovulation occur?
~24 hours
What prevents a dominant follicle from ovulating?
Presence of a dominant corpus luteum
How many follicular waves does it typically take until a follicle ovulates?
2 or 3
How large do follicles need to be to ovulate?
Over 10mm
Ultrasound appearance of the ovary when in follicular phase?
Large follicle and no real CL
What is ovsynch?
Synchronisation creates predictable window for AI
* Day 0: GnRH given to cause LH surge and initiate follicular waves
* Day 7: Cow given prostaglandin to lyse dominant CL and allow dominant follicle to grow
* Day 9: GnRH given to cause LH surge and allow dominant follicle to ovulate
What can be added to ovsynch and why?
Progesterone releasing device like CIDR/PRID
* Same as OvSynch except progesterone releasing device inserted on day 1, and removed on day 8
* Device acts like a CL so if there isn’t one present in the animal you still get a “luteolysis” effect and a progesterone drop
What synch protocol still requires oestrus observation
Progestogen + PGF
* Day 0: Insert progesterone device
* Day 6: Inject PGF2a
* Day 7: remove progesterone device
* Oestrus occurs 1-3 days after. Serve 12 hours into oestrus
Use of GnRH in farm work?
Induces a LH surge and ovulation 28 hours later
Use of FSH in cattle?
Stimulates antral follicle growth
Used in super-ovulation
Use of LH in cattle?
Not used often. Stimulates final maturation + ovulation
- Can induce or force ovulation
- Treat cystic ovaries
Use of progestogens in cattle?
Negative feedback on the HPG axis
Mimics the luteal phase so useful in synch protocols
Use of prostaglandins in cattle?
Lysis of the mature CL => progesterone decline => cow in proestrus
- Terminates luteal phase
- Induces abortion or parturition
- Also ecbolic effects on uterus
Examples of prostaglandins in cattle
- Dinoprost naturally occurring PGF2α
- Cloprostenol synthetic analogues
How long does ovulation take after GnRH?
Ovulation 28 hours later
How long does oestrus take after PGF2a?
Oestrus 3-5 days later after administration
Two methods to induce parturition
Prostaglandin
- Parturition if given after day 270.
- Calving ~45 hours after injection
Glucocorticoid excess (Dexamethasone)
- Parturition if given after day 270
High incidence of RFM with both
What is submission rate?
Cows eligible to be bred that are actually bred
What is conception rate?
Number of cows that are successfully bred
Breeding goals for cows after calving?
Breeding before 58 days in milk
Conception before 81 days in milk
Common cause of lack of return to cyclicity?
Negative energy balance