Replication of DNA - 1.2 Flashcards
1
Q
What enzymes are used on the leading strand during replication and how is it replicated?
A
-DNA polymerase
-continously
2
Q
Requirements for DNA
A
-DNA template
-primers
Free DNA nucleotides
-ATP
-Enzymes
3
Q
What are the enzymes used on the lagging strand and how is it replicated?
A
-DNA polymerase and ligase
-discontinuous
4
Q
what is a primer
A
short strand of nucleotides complementary to specific target sequences.
5
Q
Requirements for PCR
A
-sequence of DNA
-primers
-DNA nucleotides
-heat tolerant DNA polymerase
6
Q
Temperature of first stage of PCR and what happens
A
-heated to 92-98
-seperated strands and breaks hydrogen bonds
7
Q
Temperature of second stage of PCR and what happens
A
- 50-60
-primers bind to specific target sequences
8
Q
temperature of the third stage of PCR, and what happens
A
- 70-80
-heat tolerant DNA polymerase to repicate DNA region
