replication of dna 1.2

Question 1

what is replication

Answer 1

the prosess of dna molecules making an exact copy of themselves

Question 2

what enzyme replicates dna

Answer 2

dna polymerase

Question 3

what does dna polymerase beed to start replication

Answer 3

priners

Question 4

what is a primer

Answer 4

a short complimentary strand of dna nucleotides which bind to 3’ end of the dna template strand allowing dna polymerase to add dna nucleotides

Question 5

which direction doed dna polimerase work in

Answer 5

3’ to 5’

Question 6

whats the first step in replication

Answer 6

dna unwound hydrogen bonds broken forming two template strands

Question 7

what type of replication occurs on the leading strand

Answer 7

continuous

Question 8

what is replication likeon the lagging strand

Answer 8

fragmented

Question 9

how are fragments on lagging strand joined together

Answer 9

ligase enzyme

Question 10

what is pcr

Answer 10

a lab technique to amplify dna

Question 11

first step in pcr

Answer 11

heat to 92-98c to seperate dna strands

Question 12

second step in pcr

Answer 12

cooled to 50-65c to allow primers to bind to target sequence

Question 13

third step in pcr

Answer 13

heated to 70-80c for heat tolerant dna polynerase to replicate the region of dna

Question 14

whats the practical apps of pcr

Answer 14

help solve crimes
paternaty tests
diagnosis of genetic disorders

Question 15

macromolecules like dna can be seperated by

Answer 15

gel electrophoresis