Replication

Question 1

The replication fork is _________.

Answer 1

Asymmetric

Question 2

A new DNA strand is synthesized in the ________________ direction.

Answer 2

5’—to —-3’

Question 3

Describe enzyme activity of DNA polymerase at the replication fork:

Answer 3

• To replicate the lagging strand, it use a backstitching mechanism
• It synthesizes short pieces of DNA (Okazaki fragments) in the 5’ to 3’ direction and then moves back along the template strand (towards the fork) before synthesizing the next fragment.

Question 4

DNA Polymerase is___________.

Answer 4

Self Correcting.

-Its so accurate, w/ a low error rate due to the accuracy of complementary base-pairing.

Question 5

RNA primers are synthesized by __________ which uses ___________.

Answer 5

• an RNA polymerase called PRIMASE

- a DNA strand as a template