REJUVENATED RED BLOOD CELLS, WASHED RED BLOOD CELL, FROZEN > THAWED > DEGLYCEROLIZED RBCS

Question 1

 Addition of Rejuvenation solution
 The important component of RBCs are rejuvenated

Answer 1

REJUVENATED RED BLOOD CELLS

Question 2

Components revived in REJUVENATED RED BLOOD CELLS

Answer 2

ATP and 2,3 DPG

Question 3

Components added in REJUVENATED RED BLOOD CELLS (4)

Answer 3

o Phosphate
o Inosine
o Pyruvate
o Adenosine

Question 4

also used for rejuvenating RBCs but is now removed

Answer 4

Glucose

Question 5

FDA Approved Rejuvenating Solution

Answer 5

Rejuvesol

Question 6

REJUVENATED RED BLOOD CELLS SHELF LIFE

Answer 6

 Can be prepared 3 days after expiration of blood
 The addition of rejuvenating solution alters the shelf life of the blood component

Question 7

RBCs washed with sterile saline for the removal of (4)

Answer 7

plasma proteins, platelets, leukocytes and cellular debris

Question 8

Only reduce and not completely remove leukocyte

Answer 8

WASHED RED BLOOD CEL

Question 9

WASHED RED BLOOD CELL INDICATIONS (3)

Answer 9

 Anemia with history of febrile reactions
 Paroxysmal nocturnal hemoglobinuria
 For patients with plasma proteins antibodies to reduced allergic reactions (for IgA-deficient patients)

Question 10

WASHED RBC SHELF LIFE

Answer 10

Open System: 24 hours at 1-6°C

Question 11

WASHED RBC QC REQUIREMENT

Answer 11

Plasma must be removed

Question 12

Frozen RBCs are prepared for those with rare units such as AB (-) individuals, Bombay phenotype, Rh phenotype and patients that undergoes autologous donation.

Answer 12

FROZEN > THAWED > DEGLYCEROLIZED RBCS

Question 13

Good for anemic patients

Answer 13

FROZEN > THAWED > DEGLYCEROLIZED RBCS

Question 14

Cryoprotective agent –

Answer 14

Glycerol

Question 15

Keeps the RBC components from being destructed when stored in low temperatures

Answer 15

Cryoprotective agent (Glycerol)

Question 16

_____________: 40% glycerol
□ Freezer requirement:

Answer 16

High Concentration
□ Freezer requirement: Mechanical freezer (- 65°C) (Preferred)

Question 17

__________: 20% glycerol
□ Requirement:

Answer 17

Low Concentration
Liquid Nitrogen (Prone formation of ice crystals that leads to cell lysis) (- 100°C)

Question 18

79 % Glycerol + Dextrose, Fructose and EDTA

Answer 18

-65°C

Question 19

FROZEN > THAWED > DEGLYCEROLIZED RBCS SHELF LIFE
 Frozen –
 Deglycerolized –

Answer 19

 Frozen – 10 years provided that cryoprotective agent is added
 Deglycerolized – 24 hours (thawed)

Question 20

wash the RBC via 3-step washing

Answer 20

Deglycerolization

Question 21

Thaw at 1-6°C, after the unit melts, proceed to deglycerolization

Answer 21

Deglycerolize/Thawed/Defrost

Question 22

Deglycerolization uses decreasing concentration of saline or
sodium chloride (starting from ___________ to __________ solution)

Answer 22

hypertonic to isotonic solution

Question 23

1st washing process

Answer 23

wash thawed RBC to 12% NaCl (Hypertonic sol’n) – eliminates glycerol without affecting the viability of RBCs

Question 24

2nd washing process

Answer 24

wash RC at 1.6% NaCl this will facilitate the complete removal of glycerol

Question 25

3rd wash

Answer 25

0.9 or 0.85% NaCl (isotonic sol’n) – red blood cells are viable for 24 hours only.