Regulation of Gene Transcription: Promoters

Question 1

Promoters

Answer 1

Contain sequences that can bind RNA polymerase + transcription factors that regulate RNA polymerase binding

Question 2

RNA polymerase binding site

Answer 2

E.coli RNA polymerase recognises and binds to DNA sequences upstream from the transcription

Question 3

RNA Polymerase binding site

Answer 3

• The -10 & -35 sequences
• Most common base at each position
• Consensus - most common base at each position
• No RNA polymerase binding site within a promoter is a perfect match to consensus

Question 4

A model for transcription

Answer 4

• RNA polymerase is recruited to promoter
• RNA-P opens DNA to form transcription bubble
• RNA-P transcribes the double stranded DNA making an RNA copy of coding strand using the template