Regulation of Gene Expression Flashcards

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1
Q

What level gene regulated?

A

1) Transcript present?
2) Transcript structure?
3) Protein expressed?

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2
Q

Transformer gene

- female development

A

Male = off Female = on

  • transcript in both
  • translated only in females
  • females = splicing –> transcript
  • males = stop codon –> no transcript
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3
Q

Regulatory Sequence

A

Bacteria

  • Positive = activator
  • Negative = operator

Eukaryote

  • Positive = enhancer
  • Negative = silencer
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4
Q

Promoter

A

Promoter = (+) prokaryote (-) eukaryote
Promoter & operator = (-) prokaryote (-) eukaryote
Promoter & activator = (+) prokaryote (+) eukaryote
Promoter, operator, activator = (-) prokaryote (-) eukaryote

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5
Q

LacZ and Laci

A

Laci has diffusible factor = trans-acting to LacZ

Operator LacZ recognize diffusible factor = cis acting to LacZ

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6
Q

Complementation Test

A

Mutations in different genes
- trans acting

Mutations in same genes
- cis acting

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7
Q

Intragenic Analysis

A
  • operator constitutive dominant to lacZ loss of function in trans
  • wild type alleles dominant when operator constitutive and lacZ loss of function in cis
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8
Q

Transcription Activators

A

binds to enhancer and silencers

- activate transcription

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9
Q

Sex Lethal Protein

A
  • blocks use of male 3’ ss
  • alternative splicing
  • only females have this protein
  • males have no sex lethal protein – stop codon
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10
Q

Nanos

A
  • suppresses HB protein expression
  • nanos null = expression
  • binds to pumilio
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11
Q

Pumilio

A
  • suppresses HB protein expression

- binds to nanos regulatory element

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