Chromosomal Mutants Flashcards
Karyotype
Metacentric – middle
Submetacentric – close to middle
Acrocentric – close to end
Telocentric – end
Chromosomal Deletions RF
no recombination in deletion
- crossovers closer together
- higher RF
Chromosome Deletions Recessive Alleles
genes lost in deletion = null loss of function alleles
Chromosome Deletion Polytene Chromosomes
bubble
- bottom strand = deletion
Chromosome Deletion PCR Analysis
primers closer together b/c deletion
- see product from deletion chromosome
Chromosome Duplications - Types
Tandem = close + direct Displaced = far + direct Reverse = close + inverted
Chromosome Duplication Polytene Chromosomes
bubble
- top strand = duplicated
Paracentric Inversion
- NOT include centromere
- centromeres pulled apart on same strand
DRAW OUT
Pericentric Inversion
- include centromere
- centromeres pulled apart on opposite strands
DRAW OUT
Effect of Inversions/Translocations
- next to new DNA
- split DNA into 2 loss of functions
- recombination in inversion = non-viable gametes
Non-Reciprocal Translocation
region of one chromosome insert into another
Reciprocal Translocation
regions of chromosomes switched btwn pair of homologous chromosomes
Reciprocal Translocations - Adjacent 1
- horizontal division
- inviable gametes = missing info
Reciprocal Translocations - Adjacent 2
- vertical division
- inviable gametes = missing info
Reciprocal Translocations - Alternate segregation
- translocated chromosomes one way
- normal chromosomes other way
- viable gametes = all info