Recommended Order for Drawing Evacuated Tubes & Filling Tubes From a Syringe Flashcards
Order of draw
Blood culture, coag (citrate), serum, heparin, EDTA, glycolytic inhibitor
stopper or closure for blood culture
yellow (SPS) or blood culture bottle
stopper or closure for coag (citrate)
light blue
stopper or closure for serum
red, gold, speckled
stopper or closure for heparin
green
stopper or closure for EDTA
lavender, pink, white
stopper or closure for glycolytic inhibitor
gray
comments on blood culture tube
drawing first avoids bacterial contamination from needle that has pierced other stoppers
comments on coag (citrate) tube
drawing before other anticoagulant and clot activator tubes avoids contamination with additives that can affect coag results
comments on serum tube
drawing before green avoids contamination with sodium or lithium heparin, K2EDTA, and sodium fluoride/potassium oxalate
Why is red, gold, speckled tubes drawn before green tubes?
sodium heparin causes ↑ Na+ and lithium heparin causes ↑ Li+
why is red, gold, speckled tubes drawn before lavender tubes?
K2EDTA causes: ↓ Ca 2+, ↓ Mg2+, ↑ K+
why is red, gold, speckled tubes drawn before gray tubes?
Sodium fluoride/potassium oxalate causes: ↓Ca2+, ↑Na+, ↑K+, and interference with some enzyme assays
comments on heparin tube
drawing before lavender avoids contamination from K2EDTA and sodium fluoride/potassium oxalate
Why is green tube drawn before lavender tubes?
K2EDTA causes: ↓Ca2+, ↓Mg2+, ↑K+
