Recombination Flashcards
Microbial recombination is
the re-arrangment of nucleic acids involving a donor cell and a recipient cell to form new genomes
Fewer plasmids in a cell means
it is harder for the bacteria cell to replicate under selection
Plasmid promiscuity is
the ability of plasmids to be transferred between different species
An incompatibility group is
groups of plasmids which are unable to co-exist within a cell
the 3 types of plasmid are
metabolic plasmids, virulence plasmids and F plasmids
metabolic plasmids encode
enzymes which break down chemicals in the bacterial enviroment or host
virulence plasmids encode
virulence genes such as adhesins, toxins, resistance to host defences
fertility factors encode
Tra genes for pili production and assembly, assembly of transferosome and relaxosome
episomes are
plasmids that can be intergrated into the bacterial chromsome or remain as a seperate plasmid
the 2 types of recombination are
homologous and site-specific
site-specific recombination describes
a recombination process in which DNA fragments with recognition sites for site specific enzymes which cut the dsDNA and the cut ends join cut ends of another duplex
homologous recombination describes
a recombination process in which nucleic acids are exchanged betwen two dsDNA regions of homology
the 2 enzymes in site-specific recombination are
recombinase and intergrase
