Recombinant technology

Question 1

What makes Type II restrictive enzymes more useful

Answer 1

They cleave where they recognize unlike other Restrictive Enzymes which cleave few nuclotides around where they recogmize

Question 2

Why are kidney patients usually anaemic

Answer 2

Because the kidney produces Erythropoietin

Question 3

When designing probes from proteins which aminoacids do you identify and use

Answer 3

Amino acid sequencds rich in methionine and tryptophan since they have only 1 codon that codes for them , making it easier to synthesize the probe

Question 4

For replication to occur what must the replication vector have

Answer 4

Origin of replication
Antibiotic Resistance Gene,
Multiple cloning sites(Poly linker regionrich with a lot of restrictive enzymes

Question 5

What must a vector have for replication

Answer 5

Antibiotic Resistance Gene,
Multiple cloning sites(Poly linker region

Question 6

What vector is mostly used in cloning

Answer 6

Plasmid

Question 7

Lentivirus

Answer 7

Needed for intro. of dna into post mitotic cells like neurons

Question 8

Intro of dna into bacteria

Answer 8

Transformation

Question 9

Intro. of dna into Eukaryotes

Answer 9

Transfectiom

Question 10

What must expression vectors have

Answer 10

Multiple cloning sites (Polylinker region- rich with a lot of restrictive enzymes
Antibiotic resitance region
Origin of replication
Promoter Region
Termination Region

Question 11

What is a plasmid vector

Answer 11

A plasmid is an extrachromosomal circular doublestranded DNA molecule that replicates autonomously in bacterial cells

Question 12

What is the major cause of cml

Answer 12

Philadelphia chromosome(chromosome 9 and 22

Question 13

find taq polymerase

Question 14

Which DNA sequencing method is the most accurate

Answer 14

Sanger (Dideoxy) Sequencing Method

Question 15

Which DNA sequencing method is used for long DNA sequences

Answer 15

Next Generation Sequencing method

Question 16

DNA micro array