Genome Editing

Question 1

What are some techniques used for Genome editing

Answer 1

Zinc Finger nucleases
Talents
CRISPR/CAS system

Question 2

What is off-target mutations and what genome editing technique caused it

Answer 2

Editing the wrong gene causing a mutation
Zinc Finger nucleases

Question 3

Which proteins are need for Spacer Acquisition

Answer 3

Cas 1 and Cas 2

Question 4

Which proteins are need for Spacer Acquisition

Answer 4

Cas 1 and Cas 2

Question 5

Which Cas operon gene is trnascribe for CRISPR

Answer 5

TYPE II

Question 6

Which RNA is needed for the maturation of the crRNA

Answer 6

tracrRNA

Question 7

What enzyme cuts the individual spacer RNA of the pre-crRNA to a mature crRNA

Answer 7

RNase III

Question 8

What are the two domains of cas9

Answer 8

RuvC and HNH Which cut the dna strands separately

Question 9

What differentiates the bacteria genome from the viral genome preventing it from being also cut buy CAS9

Answer 9

The PAM sequence

Question 10

What differentiates the bacteria genome from the viral genome preventing it from being also cut

Answer 10

The PAM sequence

Question 11

Which strands does each of the domains of Cas9 cleave

Answer 11

HNH cleaves the strand complementary to the crRNA strand and Ruv C cleaves the opposite strand