Recombinant DNA Technology

Question 1

First report of a cloning of an animal was

Answer 1

Dolly the sheep

Question 2

nucleus of sheep was entered into a ____ to form the clone embryo

Answer 2

enucleated unfertilized oocyte

Question 3

Complimentary base pairing is the basis for most of this technique

Answer 3

recombinant DNA techniques

Question 4

Using a retroviral enzyme (reverse transcriptase) to make DNA copied of mRNA

Answer 4

cDNA synthesis

Question 5

Which plasmid was the first used as a cloning vector?

Answer 5

pBR322

Question 6

What are the two resistance factors inside pBR322

Answer 6

Ampicillin and Tetracycline

Question 7

If you used Pstl as your cloning site, what will happen?

Answer 7

Your vector will no longer be ampicillin resistant

Question 8

If you used BamHI & Sal1 as your cloning site, what will happen?

Answer 8

Your vector will no longer be Tetracycline resistant

Question 9

What 3 components does a vector need?

Answer 9

R genes
Ori
RE sites

Question 10

This enzyme seals gaps

Answer 10

DNA ligase

Question 11

• Bacterial defense mechanism
• DS DNA sequence specificity, recognize 4-8 bp palindromes
• Many produce cohesive “sticky” ends (ie. SS complementary overhangs)

Answer 11

Type II Restriction Endonucleases

Question 12

EcoRI create (sticky/blunt) ends, while HaeIII create (sticky/blunt) ends

Answer 12

sticky, blunt

Question 13

This technology is useful for comparing genes expressed in 2 populations of cells or tissues

Answer 13

Microarray technology

Question 14

-FDA approved test for variants in CYP2D6 and CYO2C19 that helps predict patient response to a wide variety of drugs
1 Categorizes patients as ultra-rapid, extensive, intermediate or poor metabolizera

Answer 14

Amplichip CYP450

Question 15

This enzyme attached to the template strand to replicate it in PCR reactions

Answer 15

Taq polymerase

Question 16

This method is used to detect point mutations using allele-specific primers (ASPs)

Answer 16

ARMS PCR/Allele-specific PCR

Question 17

What characteristic of DNA is used in forensic science?

Answer 17

PCR is used to compare variable number of tandem repeats (VNTR) to compare 2 types of DNA.

Question 18

Everyones 13 short tandem repeats of DNA are stored in

Answer 18

CODIS (Combined DNA Index System)

Question 19

Microinjection of DNA directly into a fertilized egg cell

Answer 19

Transgenic animals

Question 20

If you can introduce an inactive form of a gene and homologously recombine it into the correct site in the genome, you can get a ___ which are extremely useful in developmental studies

Answer 20

Knockout

Question 21

This type of gene therapy is performed on cells that can be removed from the patient, manipulated, and returned. It is currently the most reliable form of therapy. Ashanti was the first successful case and she had this disease _____

Answer 21

Ex vivo, SCID

Question 22

Name this type of stem cell:

• From zygote
• can form whole human

Answer 22

Totipotent

Question 23

Name this type of stem cell:

• From blastocyst
• Can form cell types from all 3 germ layers

Answer 23

Pluripotent

Question 24

Name this type of stem cell:

• From adult
• Can form many closely related cell types

Answer 24

Multipotent

Question 25

- Transfer adult cell nucleus into enucleated egg - stimulate to develop into blastocyst - Harvest embryonic stem cells - Not yet successful in human cells

Answer 25

Somatic cell nuclear transfer

Question 26

- Introduce 4 transcription factor genes into adult differentiated cells - Induce them to reprogram into stem cells - Not yet successful in human cells (tumorigenic)

Answer 26

iPS cells (induced pluripotent stem cells)