RECALLS 💡 Flashcards

1
Q

modified Acid Fast stain DECOLORIZER

A

ACID ALCOHOL

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2
Q

done to recover and identify Clostridium spp from mixed populations of organisms

A

ETHANOL SHOCK TEST

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3
Q

Mycobacterium spp that can cause pulmonary infection RESEMBLING M. tuberculosis

A

Mycobacterium xenopi

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4
Q

often recovered from HOT WATER TAPS & CONTAMINATED WATER SYSTEMS

A

Mycobacterium xenopi

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5
Q

used for the detection of LIPASE and LECITHINASE PRODUCTION

A

EGG YOLK AGAR / MODIFIED Mc CLUNG’s or NEOMYCIN AGAR

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6
Q

(+) opaque precipitate within agar or IRIDESCENT SHEEN or PEARLY LAYER on the surface of the agar

A

EGG YOLK AGAR / MODIFIED Mc CLUNG’s or NEOMYCIN AGAR

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7
Q

primary PHYSICAL barrier

A

SKIN

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8
Q

contact time of IODINE ON SKIN FOR BLOOD CULTURE

A

60 SECONDS

or 30 seconds pero mas preferable ang 60 seconds

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9
Q

spirochete OFTEN DETECTED IN HEMA LAB

A

Borrelia

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10
Q

method of choice for DIAGNOSIS OF LYME DISEASE

A

SEROLOGIC TEST (IF or EIA)

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11
Q

U agar with Urea and Phenol Red is for what organism?

A

Ureaplasma urealyticum

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12
Q

medium of choice for theSELECTIVE RECOVERY OF GRAM (-) ANAEROBES

A

KANAMYCIN-VANCOMYCIN LAKE BLOOD (KVLB)

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13
Q

Mycobacterium isolated FROM SUBCUTANEOUS SKIN (+) GROWTH at 30 degC

A
  1. Mycobacterium marinum
  2. Mycobacterium ulcerans
  3. Mycobacterium haemophilum
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14
Q

ON XLD:

  1. COLORLESS COLONIES
  2. RED COLONIES WITH BLACK CENTERS
  3. YELLOW COLONIES
A
  1. Shigella
  2. Salmonella
  3. [EKE]
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15
Q

common laboratory acquired infections

A
  1. HEPATITIS
  2. SALMONELLOSIS
  3. TB
  4. SHIGELLOSIS
  5. BRUCELLOSIS
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16
Q

MOST IMPORTANT practice to prevent spread of infection

A

HANDWASHING

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17
Q

most susceptible to infection

A

WARM MOIST SKIN

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18
Q

MOST COMMON method of TREATING INFECTIOUS WASTE

A

INCINERATION

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19
Q

CSF and blood should always be kept at what temperature?

A

35-37°C

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20
Q

variables that may change the MIC

A
  1. INOCULUM SIZE
  2. INCUBATION TIME
  3. GROWTH RATE OF BACTERIA
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21
Q

members of Enterobacteriaceae that MOST COMMONLY PRODUCE ESBL

A
  1. E. coli
  2. K. pneumoniae
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22
Q

the ONLY NON-FERMENTER that produces ABUNDANT H2S on KIA or TSI

A

Shewanella putrefaciens

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23
Q

M agar with Arginine and Phenol Red is for what bacteria?

A

Mycoplasma

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24
Q

M. tuberculosis cultures are held in the lab after identification and AST for how long?

A

6 MONTHS

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25
Q

BACKUP BROTH is also called

A

SUPPLEMENTAL BROTH

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26
Q

LIM BROTH is for what organism?

A

S. agalactiae

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27
Q

what DOH memorandum number requires 2 SPX IN 1 DAY FOR TB DETECTION?

A

DOH MEMORANDUM NO. 2023-0021

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28
Q

In DOH Memo No. 2023-0021, how many sputum specimens are to be collected for TB detection?

A

2 SPX IN 1 DAY

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29
Q

plan that identifies tasks that are hazardous to employees and promote employee safety

A

EXPOSURE CONTROL PLAN

30
Q

the Exposure Control Plan is the responsibility of who?

A

MICROBIOLOGY SUPERVISOR & LAB DIRECTOR (CMT)

31
Q

flexible calcium alginates nasopharyngeal swab is the device of choice for…?

A

Corynebacterium diphtheriae

32
Q

CAP can provice X and V factor for…?

A

Haemophilus

33
Q

CCFA with neutral red will make colonies of C. difficile what color?

A

YELLOW

because of FRUCTOSE FERMENTATION

34
Q

VP test detects which product of glucose fermentation?

A

ACETOIN or ACETYL METHYL CARBINOL

35
Q

amine product of ORNITHINE DECARBOXYLATION

A

PUTRESCINE

36
Q

organisms that may produce YELLOW SLANT ON TSI but RED SLANT ON KIA

A
  1. Citrobacter
  2. Serratia
  3. Edwardsiella
37
Q

MOST COMMONLY IMPLICATED in DIARRHEAL DISEASE

A
  1. Escherichia
  2. Salmonella
  3. Shigella
  4. Yersinia
38
Q

produces WRINKLED COLONIES but differentiated thru biochemical tests

A
  1. Pseudomonas stutzeri
  2. Burkholderia pseudomallei
39
Q

CETRIMIDE AGAR is for the ID of what organism?

A

Pseudomonas aeruginosa

40
Q

CETRIMIDE AGAR is aka…

A

ACETYL TRIMETHYL AMMONIUM BROMIDE AGAR

41
Q

Pyocyanin (blue pigment) of P. aeruginosa can be noted on what medium?

42
Q

what is used to differentiate NON-PATHOGENIC Moraxella growing on selected media for Neisseria from Neisseria species?

A

10 UNITS OF PENICILLIN DISKS

43
Q

Gram (+) slender rods GROWING ON TOMATO JUICE AGAR

A

Lactobacillus

44
Q

MANGANOUS CHLORIDE UREA TEST is rapid test for what organism?

A

U. urealyticum

45
Q

resistance of N. gonorrhoeae to Penicillin is due to

A

BETA LACTAMASE

46
Q

collection of urine from px with INDWELLING CATHETER

A

ASPIRATE ASEPTICALLY FROM THE CATHETER TUBING

47
Q

AST method RECOMMENDED TO DETECT VANCOMYCIN INTERMEDIATE S. aureus

A

MIC BROTH DILUTION METHOD

48
Q

effect of placing antibiotic disk after organism inoculation and incubating within 15 minutes after disk placement

A

DECREASES ZONE DIAMETER

49
Q

MISTAKEN FOR N. gonorrhoeae in gram-stained smears from UTERINE CERVIX EXUDATE

A

Moraxella osloensis

50
Q

antimicrobial combination useful in CONFIRMING PRESENCE OF ESBL IN E. coli

A

CEFTAZIDIME nad CLAVULANIC ACID

51
Q

yeast enzyme detected by BIRDSEED AGAR

A

PHENOL OXIDASE

52
Q

DO NOT FORM PSEUDOHYPHAE OR ARTHROCONIDIA; produces only Blastoconidia

A

Cryptococcus

53
Q

produces Pseudohyphae and Blastoconidia; will NOT PRODUCE ARTHROCONIDIA

54
Q

produces PSEUDOHYPHAE, BLASTOCONIDIA, and ATHROCONIDIA

A

Trichosporon

55
Q

produce PSEUDOHYPHAE and ARTHROCONIDIA

A

Geotrichum

56
Q

produce ASCOSPORES

A

Saccharomyces cerevisiae

57
Q

a rare cause of Cat Scratch Disease

A

AFIPIA FELIS

58
Q

test of choice for the **CONFIRMATION OF C. trachomatis ** INFECTION IN URINE

A

PCR MOLECULAR TESTING

59
Q

the MOST RELIABLE for the detection of Mycoplasma pneumoniae in serum and for the CONFIRMATION OF DIAGNOSIS

A

EIA and DIRECT ANTIGEN TESTING

60
Q

features in which MOLDS are routinely categorized

A
  1. MACROSCOPIC GROWTH CHARACTERISTICS
  2. MICROSCOPIC MORPHOLOGY
61
Q

MOST SENSITIVE TEST for the initial dx of cryptococcal disease

A

CRYPTOCOCCAL ANTIGEN

62
Q

microscopic structures that are useful in the identification of DERMATOPHYTES

A
  1. MACROCONIDIA
  2. MICROCONIDIA
63
Q

most useful in distinguishing yeast morphology

A

CORN MEAL AGAR WITH TWEEN 80

64
Q

encapsulated fungus associated with Eucalyptus tree

A

Cryptococcus gattii

65
Q

spx of choice for RSV DETECTION

A

NASOPHARYNGEAL ASPIRATE

66
Q

**MOST COMMON ANAEROBIC BACTERIA ** isolated from infectious processes of SOFT TISSUES and BACTEREMIA

A

Bacteroides fragilis

67
Q

MOST SENSITIVE for the recovery of Brucella in CHRONIC INFECTION

A

BONE MARROW

68
Q

ACCEPTABLE anticoagulants for Blood, BM, and Synovial Fluid that are to be cultured

A
  1. SPS
  2. HEPARIN
69
Q

UNIVERSALLY SUSCEPTIBLE TO PENICILLIN

A

S. pyogenes

70
Q

primary marker of FECAL CONTAMINATION