RECALLS 💡 Flashcards
modified Acid Fast stain DECOLORIZER
ACID ALCOHOL
done to recover and identify Clostridium spp from mixed populations of organisms
ETHANOL SHOCK TEST
Mycobacterium spp that can cause pulmonary infection RESEMBLING M. tuberculosis
Mycobacterium xenopi
often recovered from HOT WATER TAPS & CONTAMINATED WATER SYSTEMS
Mycobacterium xenopi
used for the detection of LIPASE and LECITHINASE PRODUCTION
EGG YOLK AGAR / MODIFIED Mc CLUNG’s or NEOMYCIN AGAR
(+) opaque precipitate within agar or IRIDESCENT SHEEN or PEARLY LAYER on the surface of the agar
EGG YOLK AGAR / MODIFIED Mc CLUNG’s or NEOMYCIN AGAR
primary PHYSICAL barrier
SKIN
contact time of IODINE ON SKIN FOR BLOOD CULTURE
60 SECONDS
or 30 seconds pero mas preferable ang 60 seconds
spirochete OFTEN DETECTED IN HEMA LAB
Borrelia
method of choice for DIAGNOSIS OF LYME DISEASE
SEROLOGIC TEST (IF or EIA)
U agar with Urea and Phenol Red is for what organism?
Ureaplasma urealyticum
medium of choice for theSELECTIVE RECOVERY OF GRAM (-) ANAEROBES
KANAMYCIN-VANCOMYCIN LAKE BLOOD (KVLB)
Mycobacterium isolated FROM SUBCUTANEOUS SKIN (+) GROWTH at 30 degC
- Mycobacterium marinum
- Mycobacterium ulcerans
- Mycobacterium haemophilum
ON XLD:
- COLORLESS COLONIES
- RED COLONIES WITH BLACK CENTERS
- YELLOW COLONIES
- Shigella
- Salmonella
- [EKE]
common laboratory acquired infections
- HEPATITIS
- SALMONELLOSIS
- TB
- SHIGELLOSIS
- BRUCELLOSIS
MOST IMPORTANT practice to prevent spread of infection
HANDWASHING
most susceptible to infection
WARM MOIST SKIN
MOST COMMON method of TREATING INFECTIOUS WASTE
INCINERATION
CSF and blood should always be kept at what temperature?
35-37°C
variables that may change the MIC
- INOCULUM SIZE
- INCUBATION TIME
- GROWTH RATE OF BACTERIA
members of Enterobacteriaceae that MOST COMMONLY PRODUCE ESBL
- E. coli
- K. pneumoniae
the ONLY NON-FERMENTER that produces ABUNDANT H2S on KIA or TSI
Shewanella putrefaciens
M agar with Arginine and Phenol Red is for what bacteria?
Mycoplasma
M. tuberculosis cultures are held in the lab after identification and AST for how long?
6 MONTHS
BACKUP BROTH is also called
SUPPLEMENTAL BROTH
LIM BROTH is for what organism?
S. agalactiae
what DOH memorandum number requires 2 SPX IN 1 DAY FOR TB DETECTION?
DOH MEMORANDUM NO. 2023-0021
In DOH Memo No. 2023-0021, how many sputum specimens are to be collected for TB detection?
2 SPX IN 1 DAY
plan that identifies tasks that are hazardous to employees and promote employee safety
EXPOSURE CONTROL PLAN
the Exposure Control Plan is the responsibility of who?
MICROBIOLOGY SUPERVISOR & LAB DIRECTOR (CMT)
flexible calcium alginates nasopharyngeal swab is the device of choice for…?
Corynebacterium diphtheriae
CAP can provice X and V factor for…?
Haemophilus
CCFA with neutral red will make colonies of C. difficile what color?
YELLOW
because of FRUCTOSE FERMENTATION
VP test detects which product of glucose fermentation?
ACETOIN or ACETYL METHYL CARBINOL
amine product of ORNITHINE DECARBOXYLATION
PUTRESCINE
organisms that may produce YELLOW SLANT ON TSI but RED SLANT ON KIA
- Citrobacter
- Serratia
- Edwardsiella
MOST COMMONLY IMPLICATED in DIARRHEAL DISEASE
- Escherichia
- Salmonella
- Shigella
- Yersinia
produces WRINKLED COLONIES but differentiated thru biochemical tests
- Pseudomonas stutzeri
- Burkholderia pseudomallei
CETRIMIDE AGAR is for the ID of what organism?
Pseudomonas aeruginosa
CETRIMIDE AGAR is aka…
ACETYL TRIMETHYL AMMONIUM BROMIDE AGAR
Pyocyanin (blue pigment) of P. aeruginosa can be noted on what medium?
MHA
what is used to differentiate NON-PATHOGENIC Moraxella growing on selected media for Neisseria from Neisseria species?
10 UNITS OF PENICILLIN DISKS
Gram (+) slender rods GROWING ON TOMATO JUICE AGAR
Lactobacillus
MANGANOUS CHLORIDE UREA TEST is rapid test for what organism?
U. urealyticum
resistance of N. gonorrhoeae to Penicillin is due to
BETA LACTAMASE
collection of urine from px with INDWELLING CATHETER
ASPIRATE ASEPTICALLY FROM THE CATHETER TUBING
AST method RECOMMENDED TO DETECT VANCOMYCIN INTERMEDIATE S. aureus
MIC BROTH DILUTION METHOD
effect of placing antibiotic disk after organism inoculation and incubating within 15 minutes after disk placement
DECREASES ZONE DIAMETER
MISTAKEN FOR N. gonorrhoeae in gram-stained smears from UTERINE CERVIX EXUDATE
Moraxella osloensis
antimicrobial combination useful in CONFIRMING PRESENCE OF ESBL IN E. coli
CEFTAZIDIME nad CLAVULANIC ACID
yeast enzyme detected by BIRDSEED AGAR
PHENOL OXIDASE
DO NOT FORM PSEUDOHYPHAE OR ARTHROCONIDIA; produces only Blastoconidia
Cryptococcus
produces Pseudohyphae and Blastoconidia; will NOT PRODUCE ARTHROCONIDIA
Candida
produces PSEUDOHYPHAE, BLASTOCONIDIA, and ATHROCONIDIA
Trichosporon
produce PSEUDOHYPHAE and ARTHROCONIDIA
Geotrichum
produce ASCOSPORES
Saccharomyces cerevisiae
a rare cause of Cat Scratch Disease
AFIPIA FELIS
test of choice for the **CONFIRMATION OF C. trachomatis ** INFECTION IN URINE
PCR MOLECULAR TESTING
the MOST RELIABLE for the detection of Mycoplasma pneumoniae in serum and for the CONFIRMATION OF DIAGNOSIS
EIA and DIRECT ANTIGEN TESTING
features in which MOLDS are routinely categorized
- MACROSCOPIC GROWTH CHARACTERISTICS
- MICROSCOPIC MORPHOLOGY
MOST SENSITIVE TEST for the initial dx of cryptococcal disease
CRYPTOCOCCAL ANTIGEN
microscopic structures that are useful in the identification of DERMATOPHYTES
- MACROCONIDIA
- MICROCONIDIA
most useful in distinguishing yeast morphology
CORN MEAL AGAR WITH TWEEN 80
encapsulated fungus associated with Eucalyptus tree
Cryptococcus gattii
spx of choice for RSV DETECTION
NASOPHARYNGEAL ASPIRATE
**MOST COMMON ANAEROBIC BACTERIA ** isolated from infectious processes of SOFT TISSUES and BACTEREMIA
Bacteroides fragilis
MOST SENSITIVE for the recovery of Brucella in CHRONIC INFECTION
BONE MARROW
ACCEPTABLE anticoagulants for Blood, BM, and Synovial Fluid that are to be cultured
- SPS
- HEPARIN
UNIVERSALLY SUSCEPTIBLE TO PENICILLIN
S. pyogenes
primary marker of FECAL CONTAMINATION
E. coli