Real 43 Flashcards
How do membrane vesicles move?
Bud from edges of RER cisternae, move to golgi complex- may bind with each other. Can be tracked by GFP
Golgi complex
flattened, disklike, membranous cisternae with dilated rims and associated vesicles and tubules. Different compartments with
Cis face of golgi
Sorting station that sorts proteins to be shipped to ER and those to next golgi station
Trans face of golgi
proteins are segregated into different types of vesicles heading either to the plasma membrane or to various intracellular destinations
What does golgi also help build?
carbohydrate component of glycoproteins and glycolipids
What determines order of sugars inseted into oligosaccharides?
patial arrangement of the specific glycosyltransferases that come into contact with the newly synthesized protein as it moves through the Golgi stack.
vesicular transport model
Proteins shuttled through golgi stack in vesiceles
What evidence is there for the vesicle transport model that refutes the maturation model? (golgi cisternae are transient)
Products are always being made at the same locations, discrepencies have been potentially cleared with new inco (vesicles can move backwards), compositions can change over time
What serves as primary anterograde vesicle carriers?
Gogli cisternae
What do protein coats do?
Act as a mechanical device to creat vesicle out of membrane and provide a mechanism so components can be carried by vesicle
What do vesicles carry?
Cargo (secretory, lysosomal, vacuole proteins) and machinery to target and dock it to right location
COPII-Coated vesicles
Move materials forward to ERGIC and Golgi complex by selecting and ocncentrating certain components (components that come into play in pathways later, proteins that bind to cargo)
COPI-Coated vesicles
Move vesicles backward from ERGIC and Golgi to ER and from trans to cis golgi
Clathrin coated vesicles
Move materials from TGN to endosomes, lysosomes, and plant vacuoles and from plasma membrane to cytoplasmic compartments and trafficking
What are COPII-Coated vesicles also called?
ER exit sites
Sar1
a regulatory G protein that’s a part of COPII-Coat recruited to the ER membrane. Inserts itself into cytosolic leaflet of ER bilayer with binding of GTP. Recruits other polypeptides which bind to rest of structural coat. Vesicle separates
