REAGENT STRIPS Flashcards
Reagent used in glucose (multistix)
Potassium iodide
The reagent strips should be checked every ..
Beginning of the shift
Ketone principle
Sodium nitroprusside
First morning urine pH
5.0 - 6.0
Red urine with clear plasma
Myoglobinuria
Reagents in pH
Methyl red
Bromthymol blue
How many protein does Clinical proteinuria produces
30mg/dl or greater
An increase of protein in urine due to multiple myeloma
Bence jones protein
Reagents in protein (chemstrip)
Tetrachlorophenol
Purpose of measuring glucose
For diabetes mellitus
Principle for glucose
Double sequential enzyme
Reagent used in glucose (chmstrip)
Tetramethylbenzidine
Confirmatory test for glucose
Copper reduction test (clinitest)
A phenomenon which turns the result from orange stage back to green-brown color
Pass through
How to prevent pass through
Put 2 drops of urine
This is the product of fat metabolism
Ketones
3 components of ketones
Acetone
Acetoacetic acid
Beta-hydroxybutyrate
It reacts to nitroprusside in the reaction
Acetoacetic acid
It reacts to glycine
Acetone
Reagent used in blood
Tetramethylbenzidine
If the urine is red and cloudy the patient is suspected to have
Hematuria
Red urine with red plasma but clear urine
Hemoglobinuria
Principle for blood
Pseudoperoxidase activity
How much protein does the urine have
Less than 10 mg/dl per 24 hours
Also called uromodulin which is produced in DCT
Tamm-horsfall
Normal pH range
4.5 - 8.0
Protein principle
Protein error of indicator
Reagents in protein (multistix)
Tetrabromophenol blue
pH principle
Double-indicator system
Reagent for bilirubin (multistix)
Dichloroaniline diazonium salt
Reagent for bilirubin (chemstrip)
Dichlorobenzene diazonium salt
Principle for bilirubin
Diazo reaction
Principle for urobilinogen (multistix)
Ehrlichs reaction
Principle urobilinogen (chemstrip)
Azo-coupling reaction
Bilirubin is measured for
Liver dieseases
Blood is measured for
Hematuria
Hemoglobinuria
Myoglobinuria
pH is measured for
Acid-base balance
Disease in bladder during UTI
Cystitis
Kidney disease during UTi
Pyelonephritis
Bacteria oxydizes nitrate
Nitrite
Disadvantage of nitrite
Bacteria are gram-positive
Principle for nitrite
Greiss reaction
Reagent for nitrite (multistix)
P-arsanillic acid
It means the presence of WBC thus presence of bacteria
Leukocytes
Leukocyte esterase principle
Granulocyte eaterase reaction
Principle for specific gravity
Change in pK of a polyelectrolyte
Reagent for specific gravity
Bromthymol blue
End-point color of sg
Blue to yellow
Endpoint color for nitrite
Pink
Endpoint color for leukocyte esterase
Purple
Endpoint color for urobilinogen
Red
Endpoint color for ketones
Purple
Confirmatory test for bilirubin
Ictotest
Confirmtory test for ketones
Acetest tablets
Ph endpoint color
Red - yellow low ph
Green - blue high
Protein endpoint color
Blue - green
Blood endpoint color
Green - blue
Bilirubin endpoint color
Pink to violet
Endpoint glucose color
Green to brown (multistix)
Yellow - green (chemstrip)