Reagent Strips Flashcards
Reading time of Glucose and Bilirubin:
30s
Reading time of Ketones:
40s
Reading time of SG:
45s
Reagent strip with 60s reading time:
PPBUN
Protein, pH, Blood, Urobilinogen, Nitrite
Longest reading time for reagent strips:
Leukocytes (120s)
Principle of Glucose:
Double sequential enzyme (brown)
Principle of Bilirubin:
Diazo reaction (violet)
Principle of Ketones:
Sodium nitroprusside (Legal’s; Purple)
Principle of SG:
pKa change of polyelectrolyte
Principle of Protein:
Protein error of indicators (Sorensen’s) (Blue)
Principle of pH:
Double indicator system (acidic: orange; alkaline: blue)
Principle of Blood:
Pseudoperoxidase activity of Hgb (Uniform green/blue: Hgb/Mgb; speckled/spotted: intact RBCs
Principle of Urobilinigen:
Ehrlich reaction (red)
Principle of Nitrite:
Griess reaction (uniform pink)
Principle of Leukocytes:
Leukocyte esterase (purple)
Reagents of strip for SG:
— bromthymol blue
Reagents of strip for pH:
Methyl red + bromthymol blue
Reagents of strip for protein:
Tetrabrom—
Reagents of strip for Glucose:
GO, PO + KI2
Reagents of strip for Ketones:
Na nitroprusside + Glycine
Reagents of strip for Blood:
— tetramethylbenzidine
Reagents of strip for Bilirubin:
— dichloro —
Reagents of strip for Urobilinogen:
PDAB
Reagents of strip for Nitrite:
— quinoline
Reagents of strip for Leukocytes:
Indoxylcarbonic ACID ESTER