Random Mix Flashcards
What anticoagulant is in a purple top tube?
EDTA
What is in a GTT?
Heparin
What is in a RTT?
Nothing
Which tube would you use to run a CBC?
Purple Top Tube (PTT)
Which tube would you use if a lab test required serum?
Red Top Tube (RTT)
What color are heparinized crit tubes?
Red Ring & Black Ring
Give another abbreviation that means the same as HCT
PCV
What size pippette is used for a manual WBC count using a pipette?
20ul
Give the formula for a corrected WBC count?
WBC count x 100 _______________ = WBC/ul nRBC + 100
When do you need to use a corrected WBC count?
If 5 or more nRBC’s are seen per 100 diff. count
Give the name used to describe reticulocytes on a blood film stained with Wrights or Diff.
Polychromatophils
How many WBCs are counted for a WBC diff?
100
How many RBC’s are counted for a reticulocyte count?
1,000 RBCs
Give the order AND times of the Gram stain reagents
1) Crystal Violet - 1 minute
2) Grams Iodine - 1 minute
3) Decolorizer - Squirt slide, rinse w/ H2O until runs clear
4) Safranine - 1 minute
What color do Gram positive bacteria stain?
Purple
What color do Gram negative bacteria stain?
Pink
Horses & Humans are very susceptible to tetanus and should be immunized with tetanus ______?
Toxoid
Psittacosis is caused by ______?
Chlamydophilia
The coggins test is used to diagnose ______?
EIA
The gastrocnemius muscle is found on the _______?
Calf
Tendon’s connect bone to _____?
Muscle
Diff Quik Staining procedure
Fixative- 5, 1sec. dips
Eosinophilic- 5, 1sec. dips
Basophilic- 7-10, 1sec. dips
Rinse until clear
Wright’s Stain procedure
Wright’s Stain- 1 to 3min.
Buffer w/ 1 pipette wright’s- 1min.
Distilled Water- 7 to 10, 1sec. dips
Gram Stain procedure
Crystal Violet- 1min.
Gram’s Iodine- 1min.
Decolorizer- until clear
Safranin- 1min.
If a slide is stained, the condenser should be ____
Raised
If a slide is unstained, the condenser should be ____
Lowered
