Micro Flashcards
Order and times of gram stain reagents
- Crystal Violet- 1min.
- Gram’s Iodine- 1min.
- Decolorizer- Until clear
- Safranin- 1min.
What color do Gram Positive bacteria stain? And why?
Purple
The thin lipid layer is effected by the decolorizer and the pink washes away
What color do Gram Negative bacteria stain?
Pink
What 2 species are very susceptible to Tetanus and should be immunized
Humans & Horses
The Coggin’s Test is used to diagnose ___
EIA (Equine Infectious Anemia)
What is used for a Catalase Test?
Hydrogen Peroxide
A positive Catalase Test has what reaction? Meaning it is _____
Bubbles Staphylococcus
A negative Catalase Test has what reaction? Meaning it is _____
None Streptococcus
A positive Coagulase test will have what reaction? Meaning it is ____
Plasma will clot Pathogenic Staphylococcus
A Coagulase test is done on which bacteria?
Gram pos. cocci that have tested positive on the Catalase test
A negative Coagulase test will have what reaction? Meaning it is ____
No change Nonpathogenic Staphylococcus
What is Beta hemolysis? What bacteria are indicated by this reaction?
Complete lysis of RBC’s in blood agar Pathogenic Streptococcus
What is Alpha hemolysis? What 4 types of bacteria are indicated by this reaction?
Incomplete lysis of RBC’s in blood agar Strep.Pneumoniae Strep.Suis Strep.Bovis Strep.Equis
What is Gamma hemolysis?
No lysis of RBC’s in blood agar
MaConkeys agar selects for the growth of ____ and differentiates between ____ and ____
GNRODs Lactose and Non-Lactose fermenters Lactose= bright pink Non-Lactose= colorless
What is FIV and what causes it?
Feline Immunodeficiency Virus
Retroviridae- Lentevirus
Biting is the most common means of transmission it is frequently seen in aggressive, free-roaming, male cats
Pregnant women should avoid cleaning litter boxes due to ____
Toxoplasmosis gondi
Common name for the yeast that causes otitis in dogs?
Malassezia
What is equine strangles caused by?
Streptococcus equi
What causes circling disease?
Listeria monocytogenes
What causes Lyme disease?
a Spirochete-Borrelia burgdorferi Transmitted by tick
What causes Cat Scratch Disease?
Bartonella henselae
What causes Rocky Mountain Spotted fever?
Rickettsia ricketsii
What are the 2 types of Gram Pos. bacteria?
Staphylococcus Streptococcus
What are the 5 small Gram Pos. rods?
Corneybacterium
Listeria
Erysipelothrix
Arcanobacterium
Rhodococcus (more important in large animals)
What are the 2 large Gram Pos. rods?
Bacillus
Clostridium
What are the 4 branching Gram Pos. rods?
Actinomyces
Nocardia
Dermatophilus
Streptomyces
What is the Kirby-Bauer susceptibility test?
Test used to test certain bacteria’s resistance to certain types of antibiotics Area around disc is measured in mm Resistant= no clearing Intermediate= some clearing may or may not work Susceptible= clearing around disc- drug should work
What are 4 common bacteria known to cause mastitis?
Staph. aureus Staph. Agalactica E. cli Mycoplasma bovis