Micro Flashcards

1
Q

Order and times of gram stain reagents

A
  1. Crystal Violet- 1min.
  2. Gram’s Iodine- 1min.
  3. Decolorizer- Until clear
  4. Safranin- 1min.
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2
Q

What color do Gram Positive bacteria stain? And why?

A

Purple

The thin lipid layer is effected by the decolorizer and the pink washes away

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3
Q

What color do Gram Negative bacteria stain?

A

Pink

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4
Q

What 2 species are very susceptible to Tetanus and should be immunized

A

Humans & Horses

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5
Q

The Coggin’s Test is used to diagnose ___

A

EIA (Equine Infectious Anemia)

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6
Q

What is used for a Catalase Test?

A

Hydrogen Peroxide

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7
Q

A positive Catalase Test has what reaction? Meaning it is _____

A

Bubbles Staphylococcus

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8
Q

A negative Catalase Test has what reaction? Meaning it is _____

A

None Streptococcus

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9
Q

A positive Coagulase test will have what reaction? Meaning it is ____

A

Plasma will clot Pathogenic Staphylococcus

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10
Q

A Coagulase test is done on which bacteria?

A

Gram pos. cocci that have tested positive on the Catalase test

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11
Q

A negative Coagulase test will have what reaction? Meaning it is ____

A

No change Nonpathogenic Staphylococcus

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12
Q

What is Beta hemolysis? What bacteria are indicated by this reaction?

A

Complete lysis of RBC’s in blood agar Pathogenic Streptococcus

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13
Q

What is Alpha hemolysis? What 4 types of bacteria are indicated by this reaction?

A

Incomplete lysis of RBC’s in blood agar Strep.Pneumoniae Strep.Suis Strep.Bovis Strep.Equis

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14
Q

What is Gamma hemolysis?

A

No lysis of RBC’s in blood agar

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15
Q

MaConkeys agar selects for the growth of ____ and differentiates between ____ and ____

A

GNRODs Lactose and Non-Lactose fermenters Lactose= bright pink Non-Lactose= colorless

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16
Q

What is FIV and what causes it?

A

Feline Immunodeficiency Virus

Retroviridae- Lentevirus

Biting is the most common means of transmission it is frequently seen in aggressive, free-roaming, male cats

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17
Q

Pregnant women should avoid cleaning litter boxes due to ____

A

Toxoplasmosis gondi

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18
Q

Common name for the yeast that causes otitis in dogs?

A

Malassezia

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19
Q

What is equine strangles caused by?

A

Streptococcus equi

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20
Q

What causes circling disease?

A

Listeria monocytogenes

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21
Q

What causes Lyme disease?

A

a Spirochete-Borrelia burgdorferi Transmitted by tick

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22
Q

What causes Cat Scratch Disease?

A

Bartonella henselae

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23
Q

What causes Rocky Mountain Spotted fever?

A

Rickettsia ricketsii

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24
Q

What are the 2 types of Gram Pos. bacteria?

A

Staphylococcus Streptococcus

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25
Q

What are the 5 small Gram Pos. rods?

A

Corneybacterium

Listeria

Erysipelothrix

Arcanobacterium

Rhodococcus (more important in large animals)

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26
Q

What are the 2 large Gram Pos. rods?

A

Bacillus

Clostridium

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27
Q

What are the 4 branching Gram Pos. rods?

A

Actinomyces

Nocardia

Dermatophilus

Streptomyces

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28
Q

What is the Kirby-Bauer susceptibility test?

A

Test used to test certain bacteria’s resistance to certain types of antibiotics Area around disc is measured in mm Resistant= no clearing Intermediate= some clearing may or may not work Susceptible= clearing around disc- drug should work

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29
Q

What are 4 common bacteria known to cause mastitis?

A

Staph. aureus Staph. Agalactica E. cli Mycoplasma bovis

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30
Q

How is Malassezia diagnosed?

A

Ear swab Roll on slide Heat fix Stain with Wright’s Look for peanut men

31
Q

A. GNRODs

B. GPRODs

C.GNCocci

D.GPCocci

A

GPRODs

32
Q

A. GNRODs
B. GPRODs

C.GNCocci
D.GPCocci

A

GNRODs

33
Q

A. GNRODs
B. GPRODs

C.GNCocci
D.GPCocci

A

GPCocci

34
Q

A. GNRODs
B. GPRODs

C.GNCocci
D.GPCocci

A

GPCocci

35
Q

ID

A

Giardia Trophozyte

36
Q

ID

A

Otodectes (Ear Mite)

37
Q

ID

A

Microsporum Canis

(Ringworm)

38
Q

ID

A

Clostridium Perfringes

39
Q

ID

A

Campylobacter

40
Q

ID

A

Coccidia Isospora

41
Q

ID

A

Malassezia (Yeast)

42
Q

Rhodococcus Equi

A

Bronchopneumonia in foals

Small Gram pos. Rods

Abscess in lungs

43
Q

Bacillus Anthracis

A

Anthrax

Large Gram + Rod

Acquired by inhaling spores

44
Q

Clostridium Perfringes

A

Clostridial enterotoxemia

Large Gram + Rods

Safety pins

45
Q

Yersinia pestis

A

Gram - Rod

Causes the plague

Host= Rodents (prarie dogs)

46
Q

Bordetella bronchiseptica

A

Kennel Cough

non-enteric Gram - Rod

47
Q

Campylobacter

A

Gram - curved Rods

Usually chain linked

Scours in cattle

Wet tail in hamsters

48
Q

Mycobacterium

A

Tuberculosis

49
Q

Chlamydophila psittaci

A

Pisttacosis

“Parrot fever”

50
Q

Actinomyces Bovis

A

Lumpy Jaw

Branching Gram + Rod

Abscesses around head/neck region

51
Q

Clostridium Haemolyticum

A

Red Water Disease

Large Gram + Rod

Dark red urine

52
Q

Dermatophilus Congolensis

A

Rain Rot

Branching Gram + Rod

Likes humidity

Localized dermatitis

Lesions & scabs

53
Q

Streptococcus Equi

A

Equine Strangles

Gram + Cocci

Beta hemolysis

Enlarged lymphnodes

54
Q

Streptococcus Agalactae

A

Bovine Mastitis

Gram + Cocci

Beta

55
Q

Staph Aureus

A

MRSA

Gram + cocci

56
Q

MRSA

A

Methicillin Resistant Staph Aureus

57
Q

Corynebacterium pseduotuberculosis

A

Pigeon Fever

Small Gram + Rods

Vector= flies

58
Q

Listeria monocytogenes

A

Circling dz.

Small Gram + Rod

Acquired from contaminated food

59
Q

Clostridium botulinum

A

Limberneck in birds

Shaker foal in horses

Large Gram + Rod

60
Q

Clostridium tetani

A

Tetanus/ Lock jaw

Large Gram + Rod

Humans and horses

enther through puncture wound

61
Q

Clostridium Chauvoei

A

Black Leg

Large Gram + Rod

Liver necrosis

62
Q

Staph Hyicus

A

Greasy pig disease

Gram + Cocci

63
Q

Bartonella henselae

A

Cat Scratch Disease

Vector= fleas

64
Q

Rickettsia rickettsii

A

Rocky Mountain Spotted Fever

Vector= tick

65
Q

What is the #1 cause of ringworm in dogs & cats?

How do you diagnose?

A

Microsporum Canis

Woods lamp

66
Q

Rhabdo viridae

A

Rabies

67
Q

Retro viridae

A

FeLV

68
Q

Malassezia pachydermatis

A

Otitis

Dermatitis

69
Q

Mycoplasma haemofelis

A

Feline Infectious Anemia

Vector= Ticks/Fleas

Lives on RBCs

70
Q

EEE

A

Eastern Equine Encephalomyelitis

71
Q

E.coli is a Gram ____ Rod that commonly causes ____.

A

Negative

Cystitis

72
Q

Common name for Rhipicephalus sanguineus?

A

Brown Dog tick

73
Q

How does yeast reproduce?

A

By budding