Raman Spectroscopy Flashcards
How does Raman scattering arise?
Due to the exchange of energy between light and matter.
Light is from incident laser source. Light is inelastically scattered- shows up as shift in frequency
What is a Raman scatter?
Most scattered light is at same wavelengths (or colour) as laser source (no useful info provided- Rayleigh scatter).
Small amount of light scattered at different wavelengths (or colours) which depend on chemical structure of analyte- Raman scatter.
What causes the shift in frequency?
Energy from molecular vibrations.
What are the axis on Raman spectrum?
Y= intensity X= wavelength difference
What is a Raman spectra frequently used as?
A fingerprint
What are advantages of Raman spec?
*little sample prep
*good info provided
*aqueous samples can be used
What are disadvantages of Raman spec?
*weak effect
*fluorescence interferes
*expensive
What is Raman spec used for?
*identify compounds
*monitor reactions eg enzyme inhibition activity
*molecular and polymer interactions
