RA#2 Flashcards
What are the aerobic Gram (+) rods?
1) Bacillus anthracis
2) Bacillus cereus
3) Corynebacterium dipthriae
4) Listeria
What are the anaerobic Gram (+) rods?
Clostridium species
Which of the Gram (+) rods can produce spores?
Aerobic –> B. anthracis and B. cereus
Anaerobic –> Clostridium species
Which of the Bacillus species is motile?
B. cereus
How do you differentiate between Listeria monocytogene and Corynebacterium diphtheriae?
Listeria monocytogene is motile
What are the three Gram (+) cocci?
1) staphylococcus
2) streptococcus
3) enterococcus
What test is performed to separate Staph. from all other Gram (+) cocci?
catalase test
What test is used to distinguish S. aureus from the other Staph?
coagulase test
Which of the Staphylococcus is resistant to Novobiocin?
S. saprophyticus
Which Gram (+) cocci will be negative for catalase test?
Streptoccocus
Enterococcus
Which Gram (+) cocci is alpha hemolytic and sensitive to Optochin?
S. pneumoniae
Which Gram (+) cocci is beta hemolytic and sensitive to Bacitracin?
S. pyogenes
Which Gram (+) cocci is beta hemolytic and resistant to Bacitracin?
S. agalactiae
Which Gram (+) cocci is gamma hemolytic on blood agar test?
enteroccocus species
Which Gram (+) cocci is alpha hemolytic and resistant to Optochin?
Enterococcus species
What are the two Gram (-) cocci?
Neisseria menigitis
Neisseria gonorrhea
What test is performed to differentiate the two Neisseria?
Maltose test
Which Neisseria species is positive for maltose?
N. meningitis
What Gram (-) rods do we know?
enterobacteriaceae family
What’s the first test performed when Gram (-) rods are encountered?
lactose fermentation test
If lactose fermentation test is positive, what bacteria could it be?
Escheria coli
Klebsiell a pneumoniae
If lactose fermentation is negative, what’s next?
H2S production test/ motility test
If H2S production test/ motility test is positive, what bacteria could it be?
1) Salmonella enterica
2) Salmonella typhi
3) Proteus species
If H2S production test/ motility test is negative, what bacteria could it be?
1) Shigella species
2) Yersinia pesitis
What are the four bacteria not usually Gram stained?
1) mycoplasmas
2) mycobacteria
3) Chlamydia and Chlamydophila
4) Borrelia
What characteristics do mycoplasmas have?
lack cell wall
What characteristics do mycobacteria have?
acid fast staining
What characteristics do Chlamydia and Chlamydophila have?
obligate parasite
What characteristics do Borrelia have?
Wright’s staining