RA#2

Question 1

What are the aerobic Gram (+) rods?

Answer 1

1) Bacillus anthracis
2) Bacillus cereus
3) Corynebacterium dipthriae
4) Listeria

Question 2

What are the anaerobic Gram (+) rods?

Answer 2

Clostridium species

Question 3

Which of the Gram (+) rods can produce spores?

Answer 3

Aerobic –> B. anthracis and B. cereus

Anaerobic –> Clostridium species

Question 4

Which of the Bacillus species is motile?

Answer 4

B. cereus

Question 5

How do you differentiate between Listeria monocytogene and Corynebacterium diphtheriae?

Answer 5

Listeria monocytogene is motile

Question 6

What are the three Gram (+) cocci?

Answer 6

1) staphylococcus
2) streptococcus
3) enterococcus

Question 7

What test is performed to separate Staph. from all other Gram (+) cocci?

Answer 7

catalase test

Question 8

What test is used to distinguish S. aureus from the other Staph?

Answer 8

coagulase test

Question 9

Which of the Staphylococcus is resistant to Novobiocin?

Answer 9

S. saprophyticus

Question 10

Which Gram (+) cocci will be negative for catalase test?

Answer 10

Streptoccocus

Enterococcus

Question 11

Which Gram (+) cocci is alpha hemolytic and sensitive to Optochin?

Answer 11

S. pneumoniae

Question 12

Which Gram (+) cocci is beta hemolytic and sensitive to Bacitracin?

Answer 12

S. pyogenes

Question 13

Which Gram (+) cocci is beta hemolytic and resistant to Bacitracin?

Answer 13

S. agalactiae

Question 14

Which Gram (+) cocci is gamma hemolytic on blood agar test?

Answer 14

enteroccocus species

Question 15

Which Gram (+) cocci is alpha hemolytic and resistant to Optochin?

Answer 15

Enterococcus species

Question 16

What are the two Gram (-) cocci?

Answer 16

Neisseria menigitis

Neisseria gonorrhea

Question 17

What test is performed to differentiate the two Neisseria?

Answer 17

Maltose test

Question 18

Which Neisseria species is positive for maltose?

Answer 18

N. meningitis

Question 19

What Gram (-) rods do we know?

Answer 19

enterobacteriaceae family

Question 20

What’s the first test performed when Gram (-) rods are encountered?

Answer 20

lactose fermentation test

Question 21

If lactose fermentation test is positive, what bacteria could it be?

Answer 21

Escheria coli

Klebsiell a pneumoniae

Question 22

If lactose fermentation is negative, what’s next?

Answer 22

H2S production test/ motility test

Question 23

If H2S production test/ motility test is positive, what bacteria could it be?

Answer 23

1) Salmonella enterica
2) Salmonella typhi
3) Proteus species

Question 24

If H2S production test/ motility test is negative, what bacteria could it be?

Answer 24

1) Shigella species

2) Yersinia pesitis

Question 25

What are the four bacteria not usually Gram stained?

Answer 25

1) mycoplasmas
2) mycobacteria
3) Chlamydia and Chlamydophila
4) Borrelia

Question 26

What characteristics do mycoplasmas have?

Answer 26

lack cell wall

Question 27

What characteristics do mycobacteria have?

Answer 27

acid fast staining

Question 28

What characteristics do Chlamydia and Chlamydophila have?

Answer 28

obligate parasite

Question 29

What characteristics do Borrelia have?

Answer 29

Wright’s staining