quiz today for flow cytometry Flashcards
1
Q
Name two treatments used in the lab that may affect the cell cycle.
A
1) Serum starvation
2) Nocadazole
3) Calpain inhibitor
4) Hydrogen peroxide
2
Q
Name the major components of the flow cytometer?
A
1) Fluidic system
2) Illumation system
3) Optics + electronics
4) data storage + computer control system
3
Q
What does forward scattering tell you about a cell?
A
The size of the cell
4
Q
What does side scattering tell you about a cell?
A
Amount of granules in a cell
5
Q
What do we use in this lab to detect DNA?
A
PI, fluorescent stain/dye, GFP
