Biotechnology (W16) - Week 3,4 - Day 6 Protocol: Fixation of DNA to a membrane

Question 1

Following transfer, the position of the gel slots was marked on your membrane with a soft _____ ______.

Answer 1

lead pencil

Question 2

What was the purpose of placing the membrane in neutralization buffer and lightly shake it for 5 minutes at low speed?

Answer 2

Remove any agarose that might have adhered to the membrane, and to equilibrate the membrane.

Question 3

What was done to ensure that DNA samples do not become unattached from the membrane in downstream applications?

Answer 3

UV-Cross linking