Quiz 6 Flashcards
Types of acquired pellicle
- surface pellicle (unstained)
- surface pellicle (stained) - pellicle that has taken on extrinsic stain
- subsurface pellicle; embedded in tooth structure
Tenacious membranous layer that is amorphous, acellular, and organic. It’s thickness varies from 0.1-0.8 um and is greater near the gingival margin
Acquired pellicle
Participates in biofilm formation by aiding the adherence of microorganisms
Nidus for bacteria
Dense non mineralized complex mass of colonies in a gel like intermicrobial matrix; adheres firmly to the acquired pellicle thus to the teeth, calculus and fixed removable restorations
Dental biofilm
Stages of formation (dental biofilm)
- Formation of pellicle (bacteria attach to pellicle)
- bacterial multiplication and colonization (micro colonies form in layers)
- biofilm growth and maturation
- matrix formation (supra gingival -salvia, sub gingival- gingival sulcus fluid, exudate)
Sub gingival dental biofilm comes from…
The apical proliferation of microorganisms from the supra gingival biofilm
Sub gingival microorganisms
- differ from that of the supra gingival biofilm
- includes more anaerobic and motile organisms and are predominately gram- negative
Composition of dental biofilm
Composed of microorganisms and intermicrobial matrix
Organic and inorganic solids constitute approx
20%, 80% water
Inorganic elements in biofilm
Calcium, phosphorus, fluoride
Organic components in biofilm
Carbohydrates- from dietary sucrose, contribute to adherence of the microorganisms to each other and to the tooth
Proteins- supra gingival- contain proteins derived from salvia
Sub gingival - contains proteins derived from gingival sulcus fluid
Lipids- may include gram bacteria
During formation biofilm starts at…
Gingival margin on proximal surfaces and spears coronally
_______ surfaces may have the least biofilm because of the activity of the tongue
Palatal
Microorganism in biofilm
- mutants streptococci ( S. Mutants and Streptococuss sobrinus)
- lactobacillus
Loosely adherent mass of bacteria and cellular debris
Materia alba