Quiz 5

Question 1

In Growth Associated Product formation, product expression is linked to energy metabolism.
t/f

Answer 1

true

Question 2

All nutrients are added before inoculation

Answer 2

Batch

Question 3

Toxic waste products influence growth rate

Answer 3

Batch

Question 4

Flow rate is equal to dilution rate

Answer 4

Continuous

Question 5

Easy to maintain sterility

Answer 5

Batch

Question 6

No fluctuations in nutrients, metabolites or cell numbers in vessel

Answer 6

Continuous

Question 7

Given a time of 540min and 720min and cell count of 1.2x107 and 6.0x107 respectively:
What is the growth rate?

Answer 7

• 0.0089

(ln⁡(6.0)-ln⁡(1.2))/(720-540)=1.609/180= 0.0089

Question 8

From the Growth Rate question, what is the doubling time?

Answer 8

77.52 min

DT= ln(2)/growth rate

Question 9

Define shear damage:

Answer 9

Damage done to cells by from mechanical stress typically caused by the impellor at high speeds.

Question 10

Why is Optical Density (OD) not a reliable means of estimating cell viability?

Answer 10

Does not distinguish between living and dead cells.

Question 11

Growth rate factors include:

Answer 11

• pH
• O2
• Osmolality

Question 12

What are two advantages of using disposables in the laboratory?

Answer 12

• Less cleaning

- No cross-contamination

Question 13

Give examples of the bioreactors for the culture types listed below, do not repeat:
- Anchorage (2):

Answer 13

• Cell factories

- Rolling flasks

Question 14

Give examples of the bioreactors for the culture types listed below, do not repeat:
- Suspension (2):

Answer 14

• T-flasks,

- Spinner flasks

Question 15

Give examples of the bioreactors for the culture types listed below, do not repeat:
- Both (1):

Answer 15

• Microcarriers,

- Biofermentor

Question 16

Select the true statement(s) (more than one statement may be true):

Answer 16

• Anchorage cell culture is appropriate for most cell types

- Anchorage cells are dissociated enzymatically and mechanically

Question 17

Cell density is measured at an OD of approx. _____ nm

Answer 17

600

Question 18

In a balanced metabolic system, Oxygen Transfer Rate =

Answer 18

Oxygen Uptake Rate

Question 19

Cell Yield formula

Answer 19

Δ P
______
Δ S