Quiz 4 Flashcards
Bacterial cells have a _______________ doubling time than insect cells and a _________________doubling time than mammalian cells.
- shorter,
- shorter
n the Genentech scenario _____ cells had an overall lower protein output, but required much less downstream processing than ____cells for the production of tPa
- CHO
- E.coli
Longest period from gene introduction to protein production
Transgenic Animal
Employs use of Baculovirus
Insect
Good at producing complex proteins/products
Mammalian
Simple media requirements
Bacteria
List 4 disadvantages of serum media:
- physiological variability
- short shelf-life
- quality control difficulties
- lack of consistency and standardization
Because cell lines can change over time/generations a _______ is created to maintain the original recombinant organism. This line is later expanded into a ______ that is used for ongoing laboratory operations and production.
- Master Cell Bank (MCB)
- working cell bank (WCB)
What is the difference between defined and undefined media?
- Defined media, also known as chemically defined media is composed on specific known quantities and concentrations of chemical reagents
- Undefined media has known additives, however the specific quality and quantity of this additives is not specific/known
Order cell types from smallest (1) to largest (4):
- Bacteria
- Yeast
- Insect
- Mammalian
When performing a seed train the best time to scale to the next size is when
the cell growth is in the log phase
Cryoprotectants displace and lower the freezing point of water in cells and tissues.
t/f
true
Provide an example of each component of media listed below:
- Carbohydrate
Sugar, glucose, galactose, sucrose, lactic acid
Provide an example of each component of media listed below:
- Fatty acids/lipids
Cholesterol, fatty acids, phospholipids
Provide an example of each component of media listed below:
- Inorganic salt
K+, Na+, Ca+
