quiz 1 Flashcards
The use of living cells or the molecular components of their metabolism to produce desired products is called ______________________?
Bioprocessing
Upstream processing includes the purification and polishing of your protein product.
T/F
False
If your cell culture produces 3.00g of bioproduct/L of culture and your biofermentor has a production rate of 0.15L/hr, how much total bioproduct will you have produced in a standard 40 hour work week in grams? (show math)
3.00g . 0.15L
———- x ———— =
L . hr
= 0.45g/hr x 40 = 18g
Provide two examples of Bioprocessing products:
Wine Beer Cheese Antibiotics Vitamins Hormones Etc.
What makes the discovery phase of a new bioproduct expensive?
The time involved in research and development.
Sterilization and containment are critical in industrial scale bioprocessing operations.
T/F
True
Name 3 of the common biological materials used in Bioprocessing:
Bacteria Viruses Yeast cells Mammalian Cells Plasmids Nucleic Acids Etc.
Define Upstream and Downstream processing:
> Upstream – Recombinant DNA Technology and Cell Growth
> Downstream – Target Purification and Polishing
Genetic manipulation is the first step in bioprocessing.
T/F
True
Quality Assurance covers documentation of things like ____________ and ______________.
Product yield, purity, safety etc.
What is the name of the gene you will be working with in class?
opd
The BTEC 4101 laboratory makes use of a ______L bioreactor.
3
A pilot scale bioreactor can be as large as _____________L.
10,000
