Quiz 4 Flashcards
The agarobiose is a dimer of
Agar
In a mixture of the five proteins listed below, which protein molecule should elute FIRST in size-exclusion (gel-filtration chromatography)? [Mr= molecular weight]
LARGEST MOLECULE ELUTES FIRST
A sample of tissue from the heart of a mouse was presented to a research laboratory. In order for researchers to create a sample of single cardiac cells, they must do all of the following EXCEPT:
Agitation
Centrifugation
Use proteolytic enzymes such as Trypsin & Collagenase
Use agents to chelate Ca2+ and prevent cell-cell adhesion
Centrifugation
A cell suspension is used in experimentation, and is composed of cell types: A, B & C. The laboratory technician would like to extract the largest cell type from the suspension, what technique should he apply to the suspension? Immuno-magnetic separation Fluorescence Activated Cell Sorting Centrifugation Gentle shaking of suspension
Centrifugation
A suspension was introduced to a Fluorescence Activated Cell Sorter. Tube A was composed of single nonfluorescent cells. What charged is assigned to these cells? Negative Positive Zero None of the above
Positive
What is adjusted in FACS to ensure that most droplets have no cells? Concentration pH Both Neither
Concentration
All of the following are true in regards to Primary Cultures EXCEPT?
Finite life span
Directly from a tissue or organism
Can develop into secondary cultures by repeatedly passaging a culture condition
Infinite life span
Infinite life span
Cell strains are derived from primary cultures and cell lines by which of the following? Selection Cloning Both Neither
Both
Nonimmortalized cells eventually stop dividing and lose their ability to proliferate, this is known as Proteolytic cleavage Senescence Cell straining Suspension
Senescence
Which of the following is false in regards to the immortalization of cells?
Inhibiting active telomerase enzymes
Inactivate check point mechanisms of cell cycle
Activating oncogenes
Fusion w/ immortalized cell lines
inhibiting active telomerase enzymes
Hybridoma cell line for monoclonal antibodies production is prepared by
Activation oncogenes
Maintaining active telomerase
Fusion w/ immortal cell lines
Inactivate cell cycle check point mechanism
Fusion w/ immortal cell lines
All of the following apply to collagen coated 2D except? Soluble gradient absent Continuous layer of matrix High stiffness Soluble gradient present
Soluble gradient present
All of the following apply to collagen coated 3D except?
Soluble gradient present
Discrete matrix
Unconstrained spreading and migration in X-Y plane
Low stiffness
Unconstrained spreading and migration in X-Y plane
Cell fractionation would include which of the following techniques? Osmotic shock Freeze & thaw Blast with inert gas Ultracentrifugation
Ultracentrifugation
Solid, denser particles migrating outward toward the wall of the cylinder is referred to as Centrifugation Sedimentation Flotation Sieves
Sedimentation
