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BIO LAB > QUIZ 3 Study Guide > Flashcards

QUIZ 3 Study Guide Flashcards

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Biology 101 flashcards
1
Q

The order, substance, and purpose of each type of stain during the Gram staining procedure?

A
  1. Crystal Violet - Color Cells Purple
  2. Gram Iodine - Sets the color of the stain
  3. Ethanol/Acetone - Decolorizes; removes stain from Gram Negative bacteria
  4. Safranin - Counterstain Gram Negative cells
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2
Q

What is step 1 for the gram staining procedure?

A

Crystal Violet - Color Cells Purple

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3
Q

What is step 2 for the gram staining procedure?

A

Gram Iodine - Sets the color of the stain

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4
Q

What is step 3 for the gram staining procedure?

A

Ethanol/Acetone - Decolorizes; removes stain from Gram Negative bacteria

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5
Q

What is step 4 for the gram staining procedure?

A

Safranin - Counterstain Gram Negative cells

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6
Q

What is the bacterial cell wall is made of?

A

peptidoglycan

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7
Q

Spirillum

A

Spiral

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8
Q

Cocci

A

Spherical

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9
Q

Bacillus

A

Rods

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10
Q

What are the different shapes of bacteria?

A

Spirillum: Spiral
Cocci: Spherical
Bacillus: Rods

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11
Q

What is the purpose of the quadrant streaking technique?

A

Quadrant streaking technique isolates specific colonies

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12
Q

What is the purpose of the preparation of a
bacterial lawn?

A

Preparation of a bacterial lawn covers an entire plate

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13
Q

What part of the cell does the gram staining procedure actually stain?

A

cell wall

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14
Q

What is the procedure of the quadrant streaking technique?

A
  • Take a Bunsen burner and heat up the loop
  • pick up the sample
  • place the sample on a new dish
  • make the four quadrants
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15
Q

What is the procedure for the preparation of a
bacterial lawn?

A

Bacterial lawns can be produced manually by evenly spreading a high amount of bacteria onto an agar plate using a sterile cotton swab or a Drigalski spatula.

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16
Q

What is antiseptic?

A

An antimicrobial substance or compound. Prevents the growth of disease-causing microorganisms.

17
Q

What is a disinfectant?

A

A chemical liquid/substance that destroys bacteria.

18
Q

COlor of gram-negative v.s gram-positive

A

gram-positive organisms are either purple or blue in color, while gram-negative organisms are either pink or red in color

BIO LAB (3 decks)

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