Enzymes Study Guide

Question 1

What was the substrate of the reactions?

Answer 1

Hydrogen Peroxide

Question 2

How would the rate of reaction change when different factors changed?

Answer 2

• If the concentration of enzyme increases, the reaction rate increases
• If the temperature increases, the reaction rate increases
• Exception to this is when the temperature gets too high the proteins are irreversibly denatured and the reaction slows
• Every enzyme has an optimal pH, and changing the optimal pH can change the 3D shape of an enzyme

Question 3

What was the positive control combo:

Answer 3

Catalase and Hydrogen Peroxide

Question 4

What was the negative control combo?

Answer 4

Catalase and Water, Hydrogen Peroxide, and Water

Question 5

What is the difference between competitive and noncompetitive inhibitors?

Answer 5

• Competitive inhibitors mimic the substrate and compete for position at an active site.
• Noncompetitive inhibitors bind at an allosteric site which is different from where the substrate binds. Therefore, increasing substrate concentration would have no effect in
  reversing noncompetitive inhibition

Question 6

What did the experiments measure?

Answer 6

appearance of a product with the bubbles