Quiz 3 Flashcards
Dilution Factor
Total final volume divided by the amount added
10^-4
Dilution
Amount added divided by the total final volume
10^4
Microbistatic
Preventing the growth of microbes, stationary, inactive
Microbicidal
Chemicals that kill microorganisms
Equation of bacterial density
(Dilution Factor)(CFU-colonies)
/ amount plated
Serial Dilution
Any dilution where the concentration decreases by the same quantity in each successive step and are multiplicative
Subspecies
Culture derived from a single parent that differs in structure or metabolism from other cultures of that species
Microbial Flora
Population of microorganisms that normally reside on or in the body
Colifrom
Collection of bacterial strains that are Gram negative, rod, non-space forming and commonly found in the intestinal tract of warm-blooded animals
General purpose media
Nutrient media: to grow as wide a collection of bacterial species as possible
Selective media
Nutrient media designed to favor the growth of certain microbes and to inhibit undesirable competitors
Differential medai
Nutrient media that discriminates between groups of microorganisms on the basis of differences in their appearance due to the different chemical reactions
Colony-forming unit (CFU)
Cells of group of cells that give rise to a colony
Gastroenteritis
Inflammation of the lining of the stomach and intestine, often caused by intention
Myalgias
muscle pain
02 is gas being released on
catalase tests
SIM
Sulfide Indole Motility Active compound= Sulfur and Tryptofan Indicator: Kovacs D: H2S gas, endole production, motility Any type of bacteria Positive Result: Black participate: sulfide Red ring: endole production
MSA
Mannitol Salt Agar Active compound: Salt Indicator: Phenol Red S: Gram + D: manitol fermentation Staph aureous + yellow low pH Staph epi - red high pH Positive Result: yellow when mannitol is fermented
EMB
Eosin Methylene Blue Agar Active Compound & Indicator: Eosin and Methylene Blue S: Gram - D: lactose fermentation Positive Result: Metallic green E. Coli, E aero
TSI
Triple Sugar Iron Active Compound: glucose, sucrose, lactose, sulfur Indicator: Phenol Red D: based on sugars it metabolizes Any organisms Positive Result: pg 145
mesophiles
37 degrees, can survive in us
MAC
MacKonkey Agar
Simmon’s citrate Positive is
Blue
Positive Eurease
Bright pink
MR-VP
MR: Methyl red
Catalase Test
Hydrogen peroxide makes CO2
O2 is gas is being released
