Quiz 3 Flashcards

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1
Q

Dilution Factor

A

Total final volume divided by the amount added

10^-4

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2
Q

Dilution

A

Amount added divided by the total final volume

10^4

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3
Q

Microbistatic

A

Preventing the growth of microbes, stationary, inactive

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4
Q

Microbicidal

A

Chemicals that kill microorganisms

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5
Q

Equation of bacterial density

A

(Dilution Factor)(CFU-colonies)

/ amount plated

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6
Q

Serial Dilution

A

Any dilution where the concentration decreases by the same quantity in each successive step and are multiplicative

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7
Q

Subspecies

A

Culture derived from a single parent that differs in structure or metabolism from other cultures of that species

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8
Q

Microbial Flora

A

Population of microorganisms that normally reside on or in the body

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9
Q

Colifrom

A

Collection of bacterial strains that are Gram negative, rod, non-space forming and commonly found in the intestinal tract of warm-blooded animals

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10
Q

General purpose media

A

Nutrient media: to grow as wide a collection of bacterial species as possible

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11
Q

Selective media

A

Nutrient media designed to favor the growth of certain microbes and to inhibit undesirable competitors

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12
Q

Differential medai

A

Nutrient media that discriminates between groups of microorganisms on the basis of differences in their appearance due to the different chemical reactions

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13
Q

Colony-forming unit (CFU)

A

Cells of group of cells that give rise to a colony

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14
Q

Gastroenteritis

A

Inflammation of the lining of the stomach and intestine, often caused by intention

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15
Q

Myalgias

A

muscle pain

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16
Q

02 is gas being released on

A

catalase tests

17
Q

SIM

A
Sulfide Indole Motility
Active compound= Sulfur and Tryptofan
Indicator: Kovacs
D: H2S gas, endole production, motility
Any type of bacteria
Positive Result: Black participate: sulfide
Red ring: endole production
18
Q

MSA

A
Mannitol Salt Agar
Active compound: Salt
Indicator: Phenol Red
S: Gram +
D: manitol fermentation
Staph aureous + yellow low pH
Staph epi - red high pH
Positive Result: yellow when mannitol is fermented
19
Q

EMB

A
Eosin Methylene Blue Agar
Active Compound & Indicator: Eosin and Methylene Blue
S: Gram -
D: lactose fermentation
Positive Result: Metallic green
E. Coli, E aero
20
Q

TSI

A
Triple Sugar Iron
Active Compound: glucose, sucrose, lactose, sulfur
Indicator: Phenol Red
D: based on sugars it metabolizes
Any organisms
Positive Result: pg 145
21
Q

mesophiles

A

37 degrees, can survive in us

22
Q

MAC

A

MacKonkey Agar

23
Q

Simmon’s citrate Positive is

A

Blue

24
Q

Positive Eurease

A

Bright pink

25
Q

MR-VP

A

MR: Methyl red

26
Q

Catalase Test

A

Hydrogen peroxide makes CO2

O2 is gas is being released