Quiz 1 Flashcards
BSL 2
- Can cause disease in healthy people, but organisms are easily contained
- Limited lab access and biohazard warning signs
- Gloves, lab coat, eye protection or face shield
- Access to autoclave
BSL 1
- No health risk
- Primary barriers: none
- Secondary barriers: Open bench tops & sinks
BSL 3
- Can cause severe disease especially when inhaled
- Controlled access to lab, no unsterilized material can leave lab, clothes need decontamination
- Bio safety cabinets used for manipulation
- HIV
BSL 4
- Highly virulent microbes with extreme risk to humans
- Clothing needs to be changed for practice, everything decontaminated
- Isolated building or lab
- EBOLA
Negative air flow
Air flows in preventing contamination of the surrounding environment
Gloves, glasses, lab coat
Protect user against spills/splashes of them or someone else
Vaccination of workers
Protects from infection in the event that other protective measures fail
Foot pedal
Prevents passing of infections as the sink handle acting as a common vehicle for infections
No eating or drinking in lab
Keep potentially contaminated food and finger away from the mouth
How to dispose of
- Petri dish with fungus
- glass culture with bacteria
- needle to draw blood
- tape close & put in white buckets
- cleanse of marks/tape and put on test tube rack in back of room
- put in hard sided biohazard container (box)
Locations of
- fire extinguisher
- disinfectant
- first aid kit
- bio hazardous waste disposal
- sharps container
- by door
- wide lab benches
- top of fridge
- white buckets and test tube rack
- by door
Parfocal
Ability to maintain focus when switching between objectives
Magnification
Creation of a larger than life image
Resolution
Clarity of an image produced by a set of lenses
Contrast
Degree of difference between the lightest and darkest parts of an image
Ocular micrometer
Circular glass disc that has a series of regularly spaced markings etched onto its upper surface
How should the condenser and diaphragm be adjusted for optimum viewing?
Condenser: best at its highest point
Diaphragm: almost completely closed
Ocular
Lens closest to your eyes when looking through a microscope, 10x
How to properly clean the lenses on a microscope
- Use lint free tissues moistened with an appropriate solvent
- Use circular motion working outward
- Clean immersion oil from objective lens
Resolution formula
Resolution = .61• wavelength /
Numerical aperture
Parcentric
Centered specimens will remain in view when switching magnifications
Depth of field
Distance from lens that is in focus
How to know if something is resolvable
If distance between points is higher than it’s resolution, then yes
Nosocomial
Acquired through a health care facility
Aseptic
Free of viable microbes
Sterile
Free of all viable life forms such as viruses and prions
no growth on agar
Ubiquitous
Existing everywhere at the same time
Pathogen
A microorganism causing disease
Inoculation
Implantation of microorganisms into a culture media
Turbid
Cloudy appearance of a nutrient solution in a test tube due to growth of a microbial population
Potable
Fit to drink, clean and odor free
How would you recognize microbial growth in a liquid media? Solid media?
Liquid: cloudiness of broth
Solid: formation if discrete colonies of cells on the surface
What is a colony and it’s relationship to a cell?
- Colony: discrete collection if many thousands to millions of cells
- All thousands of cells in a colony are descended from a single cell
What additional info does a solid culture have compared to liquid?
Growth in a solid media indicates the bacterial density of the sample
Characteristics of bacterial and fungal growth
Bacterial: typically smaller, circular, white/creamy/yellow, wet
Fungal: leathery, cottony, powdery, diverse colors
Loop
To transfer bacteria from one medium to another
Needle
To transfer bacteria by stabbing it into another medium
Sterile swabs
To collect from the environment
Broth, Agar
Liquid medium Solid medium (petri dishes)
Labeling a plate
Initials, date, class time, TSA, area of swab
Formula for total magnification
Total mag= ocular mag(10x) • objective magnification
