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Bio Lab > Quiz 1 > Flashcards

Quiz 1 Flashcards

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Biology 101 flashcards
0
Q

BSL 2

A
  • Can cause disease in healthy people, but organisms are easily contained
  • Limited lab access and biohazard warning signs
  • Gloves, lab coat, eye protection or face shield
  • Access to autoclave
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1
Q

BSL 1

A
  • No health risk
  • Primary barriers: none
  • Secondary barriers: Open bench tops & sinks
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2
Q

BSL 3

A
  • Can cause severe disease especially when inhaled
  • Controlled access to lab, no unsterilized material can leave lab, clothes need decontamination
  • Bio safety cabinets used for manipulation
  • HIV
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3
Q

BSL 4

A
  • Highly virulent microbes with extreme risk to humans
  • Clothing needs to be changed for practice, everything decontaminated
  • Isolated building or lab
  • EBOLA
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4
Q

Negative air flow

A

Air flows in preventing contamination of the surrounding environment

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5
Q

Gloves, glasses, lab coat

A

Protect user against spills/splashes of them or someone else

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6
Q

Vaccination of workers

A

Protects from infection in the event that other protective measures fail

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7
Q

Foot pedal

A

Prevents passing of infections as the sink handle acting as a common vehicle for infections

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8
Q

No eating or drinking in lab

A

Keep potentially contaminated food and finger away from the mouth

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9
Q

How to dispose of

  • Petri dish with fungus
  • glass culture with bacteria
  • needle to draw blood
A
  • tape close & put in white buckets
  • cleanse of marks/tape and put on test tube rack in back of room
  • put in hard sided biohazard container (box)
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10
Q

Locations of

  • fire extinguisher
  • disinfectant
  • first aid kit
  • bio hazardous waste disposal
  • sharps container
A
  • by door
  • wide lab benches
  • top of fridge
  • white buckets and test tube rack
  • by door
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11
Q

Parfocal

A

Ability to maintain focus when switching between objectives

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12
Q

Magnification

A

Creation of a larger than life image

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13
Q

Resolution

A

Clarity of an image produced by a set of lenses

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14
Q

Contrast

A

Degree of difference between the lightest and darkest parts of an image

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15
Q

Ocular micrometer

A

Circular glass disc that has a series of regularly spaced markings etched onto its upper surface

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16
Q

How should the condenser and diaphragm be adjusted for optimum viewing?

A

Condenser: best at its highest point
Diaphragm: almost completely closed

17
Q

Ocular

A

Lens closest to your eyes when looking through a microscope, 10x

18
Q

How to properly clean the lenses on a microscope

A
  • Use lint free tissues moistened with an appropriate solvent
  • Use circular motion working outward
  • Clean immersion oil from objective lens
19
Q

Resolution formula

A

Resolution = .61• wavelength /

Numerical aperture

20
Q

Parcentric

A

Centered specimens will remain in view when switching magnifications

21
Q

Depth of field

A

Distance from lens that is in focus

22
Q

How to know if something is resolvable

A

If distance between points is higher than it’s resolution, then yes

23
Q

Nosocomial

A

Acquired through a health care facility

24
Q

Aseptic

A

Free of viable microbes

25
Q

Sterile

A

Free of all viable life forms such as viruses and prions

no growth on agar

26
Q

Ubiquitous

A

Existing everywhere at the same time

27
Q

Pathogen

A

A microorganism causing disease

28
Q

Inoculation

A

Implantation of microorganisms into a culture media

29
Q

Turbid

A

Cloudy appearance of a nutrient solution in a test tube due to growth of a microbial population

30
Q

Potable

A

Fit to drink, clean and odor free

31
Q

How would you recognize microbial growth in a liquid media? Solid media?

A

Liquid: cloudiness of broth
Solid: formation if discrete colonies of cells on the surface

32
Q

What is a colony and it’s relationship to a cell?

A
  • Colony: discrete collection if many thousands to millions of cells
  • All thousands of cells in a colony are descended from a single cell
33
Q

What additional info does a solid culture have compared to liquid?

A

Growth in a solid media indicates the bacterial density of the sample

34
Q

Characteristics of bacterial and fungal growth

A

Bacterial: typically smaller, circular, white/creamy/yellow, wet
Fungal: leathery, cottony, powdery, diverse colors

35
Q

Loop

A

To transfer bacteria from one medium to another

36
Q

Needle

A

To transfer bacteria by stabbing it into another medium

37
Q

Sterile swabs

A

To collect from the environment

38
Q

Broth, Agar

A 
Liquid medium
Solid medium (petri dishes)
39
Q

Labeling a plate

A

Initials, date, class time, TSA, area of swab

40
Q

Formula for total magnification

A

Total mag= ocular mag(10x) • objective magnification

Bio Lab (4 decks)

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