Quiz 2 Flashcards
Trismus
Inability to open mouth fully
Aerobic
Living and growing int the presence of oxygen
Anaerobic
Growing best with the absence of oxygen
Sequelae
Morbid complication that follows a disease
Colony
Macroscopic cluster of cells appearing on a solid media each arising form the multiplication of a single cell
What is the difference between a pure culture and a mixed culture?
Pure culture contains microbial species while a mixed contains multiple
How can a pure or mixed culture become contaminated? How can this be prevented?
If your loop touches something else
- Improper sterilization or airborne contaminates
- Prevent by proper aseptic techniques
What is the importance of generating isolated bacterial colonies?
Is is the only way to obtain a PURE culture to see what microbial effects the organism has
Subculture
A second-generation culture from a well established colony of organisms
Cellular morphology
Shape (rod, spirilla)
Colonial morphology
Configurations, margins, elevation
Configuration
Margin
Elevation
- Entire colony
- Colony Edge
- Look from the side
Morphology
Study of organismic structure
Arrangement
The manner in which bacterial cells associate with one another
Pleomorphism
Normal variability of cell shapes in a single species
Mordant
Chemical that fixes dye in or on cells by forming an insoluble compound and promoting retention of the dye
Streptococci
OOOOOOOOO
Staphlococci
OOOOOOO
OOOOO
OOOOOOO
OOOOO
Tetrad
OO
OO
What stains are good for negative staining?
Useful with negatively charged bacterial cells which repel the dye (dark background)
Which stains are good for simple staining?
Useful with positively charged particles (light background)
Simple Stain
Using one dye to create contrast between bacteria and the background
Differential Stain
Using multiple dyes to create contrast between bacteria and the background
What two stains are appropriate to find the shape and arrangement of bacterial species?
Negative and simple staining
Primary Stain
Crystal violet
Mordant - stain
Iodine
Decolorizer
Ethyl alcohol
Counter stain
safranin
Order of Gram stain
- Primary Stain
- Mordant
- Decolorizer
- Counterstain
Gram + is
Gram - is
- Purple
- Pink
Gram reaction
Appearance of a cell after being gram stained
Acid fast stain
- Carbol Fuscian
- Heat
- Acid Alcohol
- Methylene Blue
Endospore Stain
- Malach Ite Green
- Heat
- Water
- Safranin
Bronchoscopy
Procedure in which a long, thin tube is inserted through the mouth to examine the airway to the lungs
Etiological agent
Microbial cause of disease (pathogen)
Bilateral infiltrates
Abnormal pulmonary opacity, indicative of fluid or cells found in both lungs
Prophylactic
Any device, method or substance used to prevent disease
2 diseases cause by Acid fast bacteria and what kind of bacteria is it
MYCObacterium- has micolic acid in cell wall
- MYCObacterium
- MYCOlic acid
- ACID fast
Why is a gram stain generally done prior to an acid fast or endospore stain?
If a cell stains Gram-, there is no need for wither of the stains because those are both for gram +
