Quiz 2 Practice Questions Flashcards
Immunoglobulin light chain
VJ
TCR alpha chain
VJ
TCR beta chain
VDJ
Immunoglobulin heavy chain
VDJ
DCs vs MPs
Sim: phagocytes, leukocytes, tissue residents, degrade pathogens
Diff: DCs transport antigen from epithelia to draining LN to activate naive T cells
T cell Ag
8-25 AA peptide
PRIMARY structure recognized as non-self AFTER DEGRADATION
CD8+ T cells
activation -> activate B cell
kill infected cells by inducing apoptosis
most abundant serum Abs
GAM
IgG, IgA, IgM
Abs expressed on naive B cell
DM
IgD or IgM
somatic hypermutation
mutational mechanism resulting in production of abs that bind antigen w/higher affinity
isotype switching
results in change of constant region of heavy chain of abs, causing change in effector fxn and transport properties of abs
Incontrasttoimmunoglobulins,alpha\betaT‐cellreceptorsrecognize epitopespresentonantigens: A. carbohydrate B. lipid C. protein D. carbohydrateandlipid E. carbohydrate,lipidandprotein.
PROTEIN
WhichofthefollowingcharacteristicsiscommontobothT‐cellreceptorsandimmunoglobulins?
A. SomaticrecombinationofV,DandJsegmentsisresponsibleforthediversityofantigen‐
bindingsites.
B. Somatichypermutationchangestheaffinityofantigen‐bindingsitesandcontributesto
furtherdiversification.
C. Classswitchingenablesachangeineffectorfunction.
D. Theantigenreceptoriscomposedoftwoidenticalheavychainsandtwoidenticallight
chains.
E. Carbohydrate,lipidandproteinantigensarerecognizedandstimulatearesponse.
SOMATIC RECOMBINATION OF VDJ INCREASES FAB DIVERSITY IN TCRS AND BCRS
TCR Fab
V alpha + V beta
Most VARIABLE domains on TCR
TCRs recognize…
PEPTIDES BOUND TO MHC
CD8 T cells combat…
INTRAcellular pathogens
CD4 T cells combat…
EXTRAcellular pathogens
CD4 Th1 cells activate…
Macrophages
CD4 Th2 cells activate and are associated with
B cell activation
HIV infection
MHC I peptide-binding groove
Alpha 1 + alpha 2
CD4 binds ____ MHC II domain
Beta 2
CD8 binds ___ MHC I domain
Alpha 3
MHC I peptide processing
proteasome -> TAP 1/2 -> ER -> MHC I -> plasma membrane
MHC II peptide processing
endocytosis -> proteases -> remove CLIP from MHC II -> peptide binds MHC II -> plasma membrane
Peptide-binding motif
combo of anchor residues in peptide that are capable of binding a particular MHC haplotype
MHC restriction
specificity of TCR for particular peptide/MHC complex
Balancing selection
maintenance of variety of MHC isoforms in a population
Directional selection
replacement of older MHC isoforms with newer variants
Interallelic conversion
gene conversion b/w alleles on homologous chromosomes
Dendrites
Highly elaborated finger-like processes on activated DCs upon arriving at LNs
Immunological synapse
areaofcontactbetweenmembranesofaTcellandanantigen‐presentingcellwherea
clusteringofprotein–proteininteractionsoccur
Th2 cytokines differentiate…
B cells
T cell prolif induced by…
IL-2 binding of HIGH affinity receptor -> T cell prolif of activated T cells
IFNg transcr factor for which cell
T-bet
CD4 Th1 cell
FOR MY MEMORY: I F’n betcha won!
IL-4 transcr factor for which cell
GATA-3
CD4 Th2 cell
FOR MY MEMORY: 2345, 13
Cytokine receptors associated w/…
JAK=cytoplasmic protein kinase
STAT=transcription factor
CD8 T cells make…
IFNg, TNFa, lymphotoxin, Fas ligand
CD4 Th2 cells make…
IL-4, CD40L, TGF-b, IL-10
Signaltransducersandactivatorsoftranscription(STATs)are
Transcr factors (JAKs)
