quiz

Question 1

molecules separate due to differences in…

Answer 1

charge
mass (length)
shape

Question 2

electrophoresis is carried out in a buffer that provides some or all of the following properties

Answer 2

maintains pH
electrical conductance
protects from degradation
maintains molecules in denatured state

Question 3

two types of gel matrix

Answer 3

agrose

polyacrylamide

Question 4

when to use agrose gel matrix?

Answer 4

preferred matrix and large sized DNA

Question 5

when to use polyacrylamide gel matrix?

Answer 5

used for very small sized DNA

Question 6

buffer for DNA gels

Answer 6

TAE or TBE

Question 7

buffer for RNA gels

Answer 7

MESA or others (may contain formaldehyde to denature RNA)

Question 8

agrose concentration for BP range between 500-5000

Answer 8

1%

Question 9

a sample loading buffer (dye) contains?

Answer 9

ficoll (high density agent)

EDTA (for preservation)

dyes

Question 10

what is used to visualize the DNA/RNA

Answer 10

***ethidium bromide

other dyes/stains

radioactive/flurescent tags

Question 11

for linear molecules… distance migrated is ___ related to…

Answer 11

inversely; length

Question 12

general properties of plasmids

Answer 12

small DNA molecules
circular
extra chromosomal
self replicating
have only non-essential genes
often carry genes that give resistance to antibiotics

Question 13

TA cloning vector

Answer 13

convenient method for cloning PCR products