lab quiz 2

Question 1

what are plasmids

Answer 1

small, circular molecules of double stranded DNA that have been derived from plasmids that occur naturally in bacterial cells, widely used for gene cloning

Question 2

polymerase chain reaction

Answer 2

technique used to exponential amplify a specific target DNA sequence, allowing for the isolation, sequencing or cloning of a single sequence among many

Question 3

what kind of PCR are we doing

Answer 3

quantitative PCR with thermocyclers

Question 4

difference between cDNA and gDNA?

Answer 4

gDNA is longer because it includes introns

Question 5

when do we use cDNA?

Answer 5

protein synthesis

Question 6

when do we use gDNA?

Answer 6

study regulatory regimes

Question 7

template DNA for PCR may include

Answer 7

cDNA or gDNA

Question 8

transcription factor

Answer 8

a protein that binds to regulatory regions and helps control gene expression

Question 9

vector

Answer 9

a DNA molecule (often plasmid or virus) that is used as a vehicle to carry a particular DNA segment into a host cell as part of a cloning or recombinant DNA technique

Question 10

1st step of PCR:

Answer 10

denaturation of the template into single strands

Question 11

2nd step of PCR:

Answer 11

annealing of primers to each original strand for new strand synthesis

Question 12

3rd step of PCR:

Answer 12

extension of the new DNA strands from the primers.

Question 13

what is in a standard PCR reaction mixture

Answer 13

-buffer with MgCl2
-dNTP mix
-primer 1
-primer 2
-template DNA
-DNA polymerase (enzyme)