Quiz 1 Info Contd

Question 1

Most enzymes interacting with DNA in the …

Different sequences of DNA have … – these are what restriction enzymes recognize

Answer 1

major groove;

particular shapes

Question 2

…-… bp per turn
steps:
… cells, …, use a … solution, … to get rid of proteins and membranes (to … them out of solution)

Answer 2

10; 11; 
lyse; 
resuspend; 
basic; 
acidify; 
precipitate;

Question 3

Lyse, resuspend, basic solution, acidify to get rid of proteins and I think membranes (to precipitate them out of solution)(replaces …)
- add … to order water around DNA and … it out of solution

Answer 3

phenol;
ethanol;
precipitate

Question 4

GFP plasmid has no other … to begin with

Resuspend - brings everything … and gives …

Answer 4

bacterial DNA;
back into solution;
correct pH

Question 5

Resuspend - brings everything back into solution and gives correct pH

• through …
• lysis steps separates the … as well and prevents genomic DNA from being …, enables it to be … at subsequent steps

Answer 5

suspending buffer;
double stranded DNA;
double stranded;
fragmented

Question 6

Resuspend - brings everything back into solution and gives correct pH

• through suspending buffer
• lysis steps separates the dsDNA as well - prevents genomic DNA from being ds, enables it to be fragmented at subsequent steps
• neutralization …. out …, … and enables … to come back together

Answer 6

precipitates out;
proteins;
membranes;
plasmid DNA

Question 7

… to allow DNA precipitation

membrane is … charged and DNA is … charged

Answer 7

ethanol;
positively;
negatively

Question 8

… layer + … will have DNA precipitate

agarose separating DNA based on … and …

Answer 8

aqueous; ethanol;
shape;
size

Question 9

running DNA against … to ensure that … wasn’t changing

product should be below … bp - if its much bigger, may be … instead of RNA to make product

Answer 9

control plasmids;
size;
1200 bp;
genomic DNA

Question 10

Using urchin DNA because … are very well defined

• embryos developing … so you can get a large amount of material to work with
• early developmental stages do look similar to some mammalian developmental stages

Answer 10

early developmental steps;

synchronously;

Question 11

for the similar amino acids between human and urchin - similar … properties (e.g. size, hydrophobic, etc)
~
more variability in … sequence than … sequence, which is why someone might start with protein sequence to find coding sequence

Answer 11

biochemical;
nucleotide sequence;
aa;

Question 12

for our pcr we’re amplifying the …

- we need to know where … and … are to design primers

Answer 12

whole coding sequence;

start codons; stop codons;