Quiz 1

Question 1

project goals:

to make … with …

Answer 1

GFP plasmid;

actin

Question 2

lab safety:

things that are dangerous (e.g. …, using … to open things)

Answer 2

food;

teeth;

Question 3

DNA contains … and … segments, is involved in …

RNA has the … only

Answer 3

coding;
noncoding;
regulation;
expressed sequence

Question 4

plasmid purification:
after lysis step, plasmid will be in the …
in the resuspension step, plasmid will be …

Answer 4

lysate;

in the cells

Question 5

gel electrophoresis:
used to separate plasmid to see … and …, comparing … of those bands against original plasmid (should be …, tells us that no new DNA was added or other DNA present)

Answer 5

supercoiled;
nicked;
sizes;
same size;

Question 6

… is the only way to go from RNA to DNA

Answer 6

reverse transcriptase

Question 7

concentration from … reading and … of bands in gel

Answer 7

nanodrop;

brightness

Question 8

C1V1 = …

this is from … concentration to … concentration

Answer 8

C2V2;
stock;
working