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Gail I
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Decks in this class (17)
Quiz 1 Info
For the project we re isolating c...,
We ll take actin and put it in a ...,
Plasmid will serve as to copy act...
36
cards
Quiz 1 - Lab Safety
No or in the lab do not store for...,
No or on cell phones and no using...,
No pipetting by use mechanical pi...
9
cards
Quiz 1 Info Contd
Most enzymes interacting with dna...,
Bp per turn steps cells use a sol...,
Lyse resuspend basic solution aci...
12
cards
Quiz 1 Info Contd 2
Flame creates that draws things u...,
Dna products,
Rna already isolated from differe...
44
cards
Quiz 1 Info Contd 3
Spectrophotometer reading gives n...,
Dna absorbing at nm ratio compari...,
For the 260 280 and 260 230 ratio...
26
cards
Quiz 1
Project goals to make with,
Lab safety things that are danger...,
Dna contains and segments is invo...
8
cards
Quiz 1 PPT Notes
Why clone and make gfp fusion pro...,
Using antibodies gives us great d...,
What gives cells their shape the ...
32
cards
pipets
P1000 goes from microliters,
P200 goes from microliters,
P20 goes from to microliters
5
cards
Quiz 2 Info
Rt lab prep make and make,
Rt lab prep also have to the rna ...,
Rt lab mix reverse transcriptase ...
21
cards
Quiz 2 Info - 2
Start with urchin rna then do rev...,
That enables transcription of the...,
In pcr heat up sample to 95 degre...
22
cards
Quiz 2 Info - 3
Nucleotide mismatch can be recogn...,
Using bacteria to replicate our b...,
We re using to clone the plasmid ...
47
cards
Quiz 2 PPTs
What can go from rna to dna,
For reverse transcription 1 templ...,
For reverse transcription 3 aka 4
37
cards
Quiz 2 PPTs - contd
Pcr lab cycling steps 1 denature ...,
Pcr lab cycling steps 2 anneal de...,
Pcr lab cycling steps 3 elongate ...
42
cards
Quiz 3 Info
We did the topo ta cloning to giv...,
Pcr on bacteria from colonies tou...
27
cards
Quiz 3 PPT
Directional pcr pcr using primes ...,
Directional pcr cloning into plasmid,
This time we re cloning into an w...
31
cards
Quiz 3 - PPTs 2
How do we look at digestion resul...,
How do we look at digestion resul...,
Primer design start by identifyin...
26
cards
Quiz 3 Info 2
The easiest way to see if there s...,
If plasmid is only being cut by o...,
We ll be plating onto lb agar pla...
47
cards
More about
Biotech
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