Questions lab 3 Flashcards
1
Q
By PCR
a) we can detect viral antigens in the sample
b) we can amplify virus specific DNA fragments in the sample
c) we can extract nucleic acid from the sample
A
b) we can amplify virus specific DNA fragments in the sample
2
Q
- By PCR we amplify
a) DNA-fragments
b) RNA-fragments
c) protein-fragments
A
a) DNA-fragments
3
Q
- Which enzyme is used to eliminate the viral proteins from the sample during nucleic acid investigation
a) trypsine
b) restriction endonucleases
c) proteinase
A
c) proteinase
4
Q
- The polymerase enzyme copies
a) the complete DNA sequence
b) specific segment of the DNA
c) the complete RNA sequence
A
b) specific segment of the DNA
5
Q
- Which ingredients are needed for the PCR
a) polymerase-I, dideoxy-nucleotides, template DNA, primers
b) restriction enzyme, phenol, RNA, mononucleotides
c) Taq polimerase, dezoxy-nucleotides, template DNA, primers
A
c) Taq polimerase, dezoxy-nucleotides, template DNA, primers
6
Q
- PCR is done in
a) thermomixer
b) thermocycler
c) cyclometer
A
b) thermocycler
7
Q
- During denaturation
a) the DNA strands get separated
b) the primers get off the DNA
c) the enzyme finishes the DNA-sequence
A
a) the DNA strands get separated
8
Q
- To transform RNA to DNA we use
a) Taq-polymerase
b) RNase
c) reverse transcriptase
A
c) reverse transcriptase
9
Q
What cleans the DNA from the filter?
A
AE buffer
10
Q
The selectivity of PCR results from?
A
Primers
11
Q
The temperature graph for PCR would look?
A
Cyclical
12
Q
What happens in annealing step?
A
Primers bind to the DNA
13
Q
Taqpolymerase has an optimal temperature of..?
A
72 degrees C
