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Virology exam > Past questions 2 > Flashcards

Past questions 2 Flashcards

1
Q

What kind of theories of origin are there?

A

A.A. Cell degeneraation

A.B. Cell autonomy

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2
Q

Which of the following is direct virus detection?

A

virus isolation: EM, IF, NA hybridisation, HA

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3
Q

Where do you inject Blue tongue virus?

A

Intravenously into embryonated hen egg

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4
Q

Which area of the embryonate egg do we use for haemagglutination test?

A

Allantoic fluid

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5
Q

What does a ‘medium’ contain?

A

Salts, amino acids, carbohydrates, indicator, antibiotics and antimimetikum

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6
Q

What is Trypsin-EDTA used for?

A

Digestion between the cells of in vitro tissue culture

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7
Q

Are there cancer cell cell cultures?

A

Yes, aneuploid cell culture

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8
Q

What is a buffy coat?

A

White blood cell culture

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9
Q

Virus extraction methods?

A

A. Freezing-thawing three times
B. Ultrasound
C. Detergent

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10
Q

With what is virus concentration by precipitation possible?

A

Ammonium sulphate, PEG, alcohol

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11
Q

Are there definitely lipids in virions?

A

Definitely not, because they are found in the external layer/envelope

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12
Q

Are quasi helical viruses always enveloped?

A

Yes

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13
Q

Are icosahedron viruses enveloped?

A

No

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14
Q

What is characteristic of complex viruses?

A

No capsomere, complete symmetry (pox virus)

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15
Q

Pleomorph viruses have no nucleocapsid

A

True

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16
Q

What can we label Nucleic acid hybridisation samples with?

A

Isotopes or enzymes

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17
Q

What can we show with PCR?

A

Nucleic acids

18
Q

What is needed for sequencing?

A

Primer, template, deoxynucleotides and marked dideoxynucleotides

19
Q

What cells are formed with the fusion of myeloma and B-cells?

A

Tetraploid hybrid cells

20
Q

Non-structural proteins do not integrate into the virion

A

True

21
Q

What is budding?

A

A type of enveloped virus release

22
Q

Are mutations only a disadvantage?

A

No, they can be advantageous

23
Q

Can the external environment influence mutation?

A

Yes

24
Q

Where are there more mutations?

A

there are more in RNA viruses

25
Q

With mutation, can species-specificity change?

A

Yes

26
Q

Are clutters thanks to/as a result of Interleukins?

A

No

27
Q

Is autointerference autoimmune?

A

No

28
Q

What is the reception of structural proteins

A

Mixing of phenotypes

29
Q

Do two attenuated vaccines activate each other?

A

Yes

30
Q

Does intramolecular recombination only happen in DNA viruses?

A

No

31
Q

Can intramolecular recombination happen in vacuoles and cytoplasm

A

Yes

32
Q

What causes cell cracking?

A

Depolarisation of the cytoskeleton

33
Q

How can you send samples?

A

Via courier, with exact legible details, +4°C, exact address

34
Q

What vectors spread rabbit haemorrhage fever?

A

Mosquitoes

35
Q

What infects bees?

A

A. Dicistroviridae (Aparavirus: acute paralysis, acute Israeli paralysis, Kashmar paralysis, Cripavirus: black mother-pearl virus)
D. Iflaviridae family (larvae cystic and wing deformity virus)

36
Q

In horses, what causes encephalitis?

A

A. Togaviridae - alphavirus genus: Eastern, Western, Venezuelan horse encephalitis, Getah virus
E. Flaviviridae - flavivirus genus: Japanese encephalitis, St. Louis encephalitis
F. Paramyxoviridae + Paramyxovirinae - Henipavirus: Hendra virus
G. Bornaviridae - Borneo virus: Bornean sickness

37
Q

All Bunyaviridae, are arbo viruses

A

False

38
Q

After taking samples, the samples have to be sent to the lab within how many hours?

A

Usually 24 hours (leukocytes: 6 hours)

39
Q

When are swab samples used?

A

With body fluid sample

40
Q

Does Border disease cause abortion in sheep?

A

Yes

41
Q

What form does herpes latency appear as in the nucleus?

A

Episome (ganglion, gland, macrophage, lymphocyte)

Virology exam (6 decks)

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