Quantitative Fluorescent PCR

Question 1

A different technique that, unlike DNA fingerprinting, is carried out in diagnostic laboratories but that again involves the analysis of tetranucleotide repeats is ____.

Answer 1

quantitative fluorescent PCR (QF - PCR)

Question 2

In this technique, selected tetranucleotide repeats are amplified by PCR using fluorescent primers and the products analyzed on an automated DNA sequencer.

Answer 2

quantitative fluorescent PCR (QF - PCR)

Question 3

Quantitative Fluorescent PCR is used as a rapid means of prenatally detecting trisomy of which chromosomes?

Answer 3

21, 18, and 13

Question 4

In Quantitative Fluorescent PCR, several markers are used for each chromosome, why is this?

Answer 4

Some markers will be uninformative if the maternal and paternal alleles contain the same number of tetranucleotide repeats at that locus.

Question 5

In Quantitative Fluorescent PCR, signal intensities of the chromosome - specific marker PCR products are compared. Trisomies are detected by the presence of ____, representing three alleles instead of two or by a ____.

Answer 5

three peaks representing a trialllelic pattern instead of two; trisomic diallelic pattern, i.e. two peaks with a 2:1 size ratio