DNA Fingerprinting

Question 1

This tandem repeats are usually 1-3 kb in length and have longer repeat motifs.

Answer 1

minisatellite

Question 2

Minisatellite show marked variation in repeat number so that about ____of individuals are heterozygous for each polymorphism.

Answer 2

70%

Question 3

Minisatellite polymorphism analysis involves Southern blotting using probes that simultaneously detect multiple minisatellite loci resulting in a ____ that consists of multiple bands of different sizes that are specific to an individual.

Answer 3

DNA fingerprint

Question 4

Minisatellite polymorphism analysis may also be conducted using ____ with greater sensitivity and requires much smaller quantities of DNA.

Answer 4

PCR

Question 5

Half of the bands in a DNA fingerprint are inherited from each parent and thus DNA fingerprinting has been widely used for _____, for ____, and for _____.

Answer 5

disputed paternity cases; resolving family relationships in immigration disputes; forensic identification of tissue samples at crime scenes

Question 6

On account of technological improvements, the preferred method for forensic genetic fingerprinting is now the analysis of _____, w/c on account of their smaller size compared w/ minisatellites, can be analysed by PCR rather than by Southern blotting.

Answer 6

multiple (at least ten) highly polymorphic, single - locus, tetranucleotide repeat microsatellites

Question 7

The ____ in the UK now contains well over 5,000,000 DNA profiles and has permitted the identification in the past year alone, of over 11,000 suspects by the identification of matches between these profiles and crime scene DNA samples.

Answer 7

National DNA Database (NDNAD)