Next Generation Sequecing Flashcards
In this type of sequencing, millions of chains of nucleotides are elongated simultaneously upon a single surface or chamber, with high-resolution image capture and sophisticated processing.
Massively parallel/Next Generation Sequencing
In this type of NGS, DNA is attached to agarose beads within emulsion droplets.
Pyrosequencing
New sequencing technologies involves electrophoretically pulling single DNA molecules through microscopic holes know as ____.
nanopores
In nanopore sequencing, if an electric charge is applied to the nanopore itself, then, as different nucleotides pass through, the DNA sequence can be determined from the distinguishable, although minute, sizes of the ____ that flows.
current
Nanopore sequencing can generate an enormous quantity of sequence data at ___ per day.
2000 MB or 2 GB
Nanopore sequencing, allowing the analysis of the entire coding regions of multiple genes all at the same time, would be of particular value for conditions that exhibit marked ____ ,such as ____, ____, and ____.
locus heterogeneity; hypertrophic cardiomyopathy; deafness; retinitis
In NGS, DNA of several patients can be pooled and analysed simultaneously, provided that each patient ’ s DNA fragments are all molecularly bar - coded or tagged with a ____, allowing that patient ’ s DNA sequence data to be extracted subsequently by the associated software.
short synthesized nucleotide sequence
What are the current disadvantages of NGS?
Machine for NGS are currently expensive
Lengths of DNA that NGS machines can read are often much shorter than with current Sanger dideoxy sequencing
Necessitates sequence assembly, w/c can be difficult in repetitive or rearranged regions of the genome
NGS has higher error rate in the raw sequence data than with the Sanger method, although this is more than off set by?
The ability to sequence in great depth, i.e., to sequence the same region many times
