Qs 81-90

Question 1

Separation solution density is:

Answer 1

1,077-1,079g/cm3

Question 2

Separation of leukocytes:

Answer 2

-Immunological non-specific
-Immunological specific

Question 3

Granularity of cells in flow cytometry are detected by?

Answer 3

Side scatter

Question 4

Parameters measures by FACS:

Answer 4

-Size of cell
-Granularity
-Intensity of fluorescence
-Identification of immune cells

Question 5

Disadvantage of IFA:

Answer 5

Expensive

Question 6

ELISA techniques are divided to what two groups?

Answer 6

-Detection of antigen : sandwich and competitive
-Detection of antibody : blocking and direct

Question 7

Disadvantage of EIA?

Answer 7

False positive

Question 8

Advantage of EIA?

Answer 8

-Detection of Ag and AB
-Very flexible method

Question 9

Application of EIS can be used for?

Answer 9

Qualitative and quantitative determination

Question 10

What does ELISA stand for?

Answer 10

Enzyme Linked Immunosorbent Assay