Qs 51-60 Flashcards
Agglutination reaction is reaction between:
Corpuscular antigen and polyclonal antibody.
Serological reaction is:
Study of blood serum where antigen binds to antibody.
-ag+ab –> agab
FACS is used for:
Analysis and sort a mixture of the cells based on their differential ability to bind fluorescence dye labeled ATB.
The direct IFA is used for:
Determination of antigen in a well or tissue by direct labelling with antibody labelled with FITC.
Phagocytosis is property of:
neutrophils, monocytes/macrophages
If we use separating solution for isolation of mononuclear cells, we need:
gently overlay separation solution, with blood and then centrifuge the sample (2500rpm/15min)
Common characteristic of separating solution for isolatopn of mononuclear cells is:
Density.
Mononuclear cells are:
Monocytes
Lymphocytes
Macrophages
The live cells are cells:
Never stained
For checking of viability of cells we may use:
Trypan blue solution.