qPCR Flashcards

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1
Q

What does qPCR stand for?

A

Real-Time or Quantitative PCR

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2
Q

What is purpose of qPCR?

A

Amplification of DNA by polymerase chain reaction (PCR)

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3
Q

What does Probe have?

A

5’ fluorescent label (6-FAM), 3’ quencher (BHQ)

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4
Q

Where does probe attach to?

A

In the DNA template between the FWD and REV primers

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5
Q

What activity does DNA polymerase have?

A

5’ to 3’ exonuclease activity

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6
Q

What part of the probe does DNA polymerase cleave?

A

5’ fluorescent label of probe and signal intensifies when separated from quencher

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7
Q

How is DNA amplification detected?

A

fluorescence is detected when fluorescence tag and quencher are separated

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8
Q

What kind of quantification does qPCR have?

A

relative quantification, uses standard curve to calculate amount of sample amplified

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